A new thrombospondin-related anonymous protein homologue in Neospora caninum (NcMIC2-like1)

Autor(es)

Pereira, L. M. ; Candido-Silva, J. A. ; De Vries, E. ^[1] ; Yatsuda, A. P. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Utrecht, Dept Infect Dis & Immunol, Div Parasitol & Trop Vet Med, NL-3508 TD Utrecht - Netherlands

^[2] Univ Sao Paulo, Dept Anal Clin Bromatol & Toxicol, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Parasitology; v. 138, n. 3, p. 287-297, MAR 2011.
• Citações Web of Science: 12

Resumo

Neospora caninum is an Apicomplexan protozoan that has the dog as a definitive host and cattle (among other animals) as intermediate hosts. It causes encephalopathy in dogs and abortion in cows, with significant loss in worldwide livestock. As any Apicomplexan, the parasite invades the cells using proteins contained in the phylum-specific organelles, like the micronemes, rhoptries and dense granules. The aim of this study was the characterization of a homologue (denominated NcMIC2-like1) of N. caninum thrombospondin-related anonymous protein (NcMIC2), a micronemal protein previously shown to be involved in the attachment and connection with the intracellular motor responsible for the active process of invasion. A polyclonal antiserum raised against the recombinant NcMIC2-like1 functional core (thrombospondin and integrin domains) recognized the native form of NcMIC2-like1, inhibited the in vitro invasion process and localized NcMIC2-like1 at the apical complex of the parasite by confocal immunofluorescence, indicating its micronemal localization. The new molecule, NcMIC2-like1, has features that differentiates it from NcMIC2 in a substantial way to be considered a homologue dagger. (AU)

• Processo FAPESP: 06/05936-0 - Caracterizacao da proteina anonima relacionada a trombospondina 29trap-2) do protozoario neospora caninum no processo de invasao celular.
• Beneficiário: Luiz Miguel Pereira
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 05/53785-9 - Proteínas envolvidas na invasão de células hospedeiras por protozoários apicomplexas como potenciais componentes para métodos profiláticos
• Beneficiário: Ana Patricia Yatsuda Natsui
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores