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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A new thrombospondin-related anonymous protein homologue in Neospora caninum (NcMIC2-like1)

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Author(s):
Pereira, L. M. ; Candido-Silva, J. A. ; De Vries, E. [1] ; Yatsuda, A. P. [2]
Total Authors: 4
Affiliation:
[1] Univ Utrecht, Dept Infect Dis & Immunol, Div Parasitol & Trop Vet Med, NL-3508 TD Utrecht - Netherlands
[2] Univ Sao Paulo, Dept Anal Clin Bromatol & Toxicol, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Parasitology; v. 138, n. 3, p. 287-297, MAR 2011.
Web of Science Citations: 12
Abstract

Neospora caninum is an Apicomplexan protozoan that has the dog as a definitive host and cattle (among other animals) as intermediate hosts. It causes encephalopathy in dogs and abortion in cows, with significant loss in worldwide livestock. As any Apicomplexan, the parasite invades the cells using proteins contained in the phylum-specific organelles, like the micronemes, rhoptries and dense granules. The aim of this study was the characterization of a homologue (denominated NcMIC2-like1) of N. caninum thrombospondin-related anonymous protein (NcMIC2), a micronemal protein previously shown to be involved in the attachment and connection with the intracellular motor responsible for the active process of invasion. A polyclonal antiserum raised against the recombinant NcMIC2-like1 functional core (thrombospondin and integrin domains) recognized the native form of NcMIC2-like1, inhibited the in vitro invasion process and localized NcMIC2-like1 at the apical complex of the parasite by confocal immunofluorescence, indicating its micronemal localization. The new molecule, NcMIC2-like1, has features that differentiates it from NcMIC2 in a substantial way to be considered a homologue dagger. (AU)