Intragenomic variation in the second internal transcribed spacer of the ribosomal DNA of species of the genera Culex and Lutzia (Diptera: Culicidae)

Autor(es)

Vesgueiro, Fabiana Tavares ^[1] ; Demari-Silva, Bruna ^[1] ; Malafronte, Rosely dos Santos ^[2] ; Mureb Sallum, Maria Anice ^[1] ; Marrelli, Mauro Toledo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Saude Publ, Dept Epidemiol, BR-01255 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Med Trop Sao Paulo, BR-01255 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Memórias do Instituto Oswaldo Cruz; v. 106, n. 1, p. 1-8, FEB 2011.
• Citações Web of Science: 18

Resumo

Culex is the largest genus of Culicini and includes vectors of several arboviruses and filarial worms. Many species of Culex are morphologically similar, which makes their identification difficult, particularly when using female specimens. To aid evolutionary studies and species distinction, molecular techniques are often used. Sequences of the second internal transcribed spacer (ITS2) of ribosomal DNA (rDNA) from 16 species of the genus Culex and one of Lutzia were used to assess their genomic variability and to verify their applicability in the phylogenetic analysis of the group. The distance matrix (uncorrected p-distance) that was obtained revealed intragenomic and intraspecific variation. Because of the intragenomic variability, we selected ITS2 copies for use in distance analyses based on their secondary structures. Neighbour-joining topology was obtained with an uncorrected p-distance. Despite the heterogeneity observed, individuals of the same species were grouped together and correlated with the current, morphology-based classification, thereby showing that ITS2 is an appropriate marker to be used in the taxonomy of Culex. (AU)

• Processo FAPESP: 05/53973-0 - Sistemática de Anopheles (Nyssorhynchus) (Diptera: Culicidae)
• Beneficiário: Maria Anice Mureb Sallum
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 05/50225-2 - Desenvolvimento de metodologias alternativas no controle de mosquitos (Diptera: Culicidae) de importância epidemiológica: uso do método ridl (liberação de insetos carregando um gene letal dominante) [... ]
• Beneficiário: Mauro Toledo Marrelli
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores