A DNA vaccine candidate encoding the structural prM/E proteins elicits a strong immune response and protects mice against dengue-4 virus infection

Autor(es)

Lima, Danielle Malta ; de Paula, Sergio Oliveira ^[1] ; de Oliveira Franca, Rafael Freitas ^[2] ; Palma, Patricia V. B. ^[3] ; Morais, Fabiana R. ^[3] ; Gomes-Ruiz, Alessandra Cristina ; Prudente de Aquino, Maria Teresa ^[2] ; Lopes da Fonseca, Benedito Antonio ^[4]

Afiliação do(s) autor(es)

^[1] Univ Fed Vicosa, Dept Gen Biol, Vicosa, MG - Brazil

^[2] Univ Sao Paulo, Sch Med Ribeirao Preto, Program Grad Studies Appl & Basic Immunol, BR-14049900 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Clin Med, Lab Flow Cytometry, BR-14049900 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Clin Med, Sch Med Ribeirao Preto, Lab Virol Mol, BR-14049900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Vaccine; v. 29, n. 4, p. 831-838, JAN 17 2011.
• Citações Web of Science: 18

Resumo

A DNA vaccine expressing dengue-4 virus premembrane (prM) and envelope (E) genes was produced by inserting these genes into a mammalian expression plasmid (pCI). Following a thorough screening, including confirmation of protein expression in vitro, a recombinant clone expressing these genes was selected and used to immunize BALB/c mice. After 3 immunizations all the animals produced detectable levels of neutralizing antibodies against dengue-4 virus. The cytokines levels and T cell proliferation, detected ex vivo from the spleen of the immunized mice, showed that our construction induced substantial immune stimulation after three doses. Even though the antibody levels, induced by our DNA vaccine, were lower than those obtained in mice immunized with dengue-4 virus the levels of protection were high with this vaccine. This observation is further supported by the fact that 80% of the vaccine immunized group was protected against lethal challenge. In conclusion, we developed a DNA vaccine employing the genes of the prM and E proteins from dengue-4 virus that protects mice against this virus. (C) 2010 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 03/07959-0 - Estudos sobre o desenvolvimento de vacinas para o virus dengue-4, transferencia adotiva da resposta imune para os virus dengue, e investigacao da co-infeccao pelos virus da dengue e febre amarela em..
• Beneficiário: Benedito Antônio Lopes da Fonseca
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 01/08523-5 - Produção de vacina de DNA recombinante para o vírus dengue-4
• Beneficiário: Danielle Malta Lima
• Modalidade de apoio: Bolsas no Brasil - Doutorado