Comparison of beta-1,3-glucanase production by Botryosphaeria rhodina MAMB-05 and Trichoderma harzianum Rifai and its optimization using a statistical mixture-design

Autor(es)

Giese, Ellen C. ^[1] ; Dekker, Robert F. H. ^[2] ; Scarminio, Ieda S. ^[3] ; Barbosa, Aneli M. ^[4] ; da Silva, Roberto ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Dept Quim & Ciencias Ambientais, Sao Jose Do Rio Preto, SP - Brazil

^[2] Lakehead Univ, Biorefining Res Initiat, Thunder Bay, ON P7B 5E1 - Canada

^[3] Univ Estadual Londrina, Dept Quim, Londrina, PR - Brazil

^[4] Univ Estadual Londrina, Dept Bioquim & Biotecnol, Londrina, PR - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Biochemical Engineering Journal; v. 53, n. 2, p. 239-243, JAN 15 2011.
• Citações Web of Science: 5
• Assunto(s): Botryosphaeria Trichoderma harzianum

Resumo

Botryosphaeria rhodina MAMB-05 produced beta-1,3-glucanases and botryosphaeran when grown on glucose, while Trichoderma harzianum Rifai only produced the enzyme. A comparison of long-term cultivation (300 h) by B. rhodina demonstrated a correlation between the formation of botryosphaeran (48 h) and its consumption (after 108 h), and de-repression of beta-1,3-glucanase synthesis when glucose was depleted from the nutrient medium, whereas for T. harzianum enzyme production commenced during exponential growth. Growth profiles and levels of beta-1,3-glucanases produced by both fungi on botryosphaeran also differed, as well as the production of beta-1,3-glucanases and beta-1,6-glucanases on glucose, lactose, laminarin, botryosphaeran, lasiodiplodan, curdlan, Brewer's yeast powder and lyophilized fungal mycelium, which were dependent upon the carbon source used. A statistical mixture-design used. to optimize beta-1,3-glucanase production by both fungi evaluated botryosphaeran, glucose and lactose concentrations as variables. For B. rhodina, glucose and lactose promoted enzyme production at the same levels (2.30 U mL(-1)), whereas botryosphaeran added to these substrates exerted a synergic effect favorable for beta-glucanase production by T. harzianum (4.25 U mL(-1)). (C) 2010 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 04/14446-1 - Producao, purificacao e caracterizacao de beta-glucanases de trichoderma herzianum para obtencao de glucooligossacarideos a partir de botriosferana.
• Beneficiário: Ellen Cristine Giese
• Modalidade de apoio: Bolsas no Brasil - Doutorado