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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Comparison of beta-1,3-glucanase production by Botryosphaeria rhodina MAMB-05 and Trichoderma harzianum Rifai and its optimization using a statistical mixture-design

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Author(s):
Giese, Ellen C. [1] ; Dekker, Robert F. H. [2] ; Scarminio, Ieda S. [3] ; Barbosa, Aneli M. [4] ; da Silva, Roberto [1]
Total Authors: 5
Affiliation:
[1] Univ Estadual Paulista, Dept Quim & Ciencias Ambientais, Sao Jose Do Rio Preto, SP - Brazil
[2] Lakehead Univ, Biorefining Res Initiat, Thunder Bay, ON P7B 5E1 - Canada
[3] Univ Estadual Londrina, Dept Quim, Londrina, PR - Brazil
[4] Univ Estadual Londrina, Dept Bioquim & Biotecnol, Londrina, PR - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Biochemical Engineering Journal; v. 53, n. 2, p. 239-243, JAN 15 2011.
Web of Science Citations: 5
Abstract

Botryosphaeria rhodina MAMB-05 produced beta-1,3-glucanases and botryosphaeran when grown on glucose, while Trichoderma harzianum Rifai only produced the enzyme. A comparison of long-term cultivation (300 h) by B. rhodina demonstrated a correlation between the formation of botryosphaeran (48 h) and its consumption (after 108 h), and de-repression of beta-1,3-glucanase synthesis when glucose was depleted from the nutrient medium, whereas for T. harzianum enzyme production commenced during exponential growth. Growth profiles and levels of beta-1,3-glucanases produced by both fungi on botryosphaeran also differed, as well as the production of beta-1,3-glucanases and beta-1,6-glucanases on glucose, lactose, laminarin, botryosphaeran, lasiodiplodan, curdlan, Brewer's yeast powder and lyophilized fungal mycelium, which were dependent upon the carbon source used. A statistical mixture-design used. to optimize beta-1,3-glucanase production by both fungi evaluated botryosphaeran, glucose and lactose concentrations as variables. For B. rhodina, glucose and lactose promoted enzyme production at the same levels (2.30 U mL(-1)), whereas botryosphaeran added to these substrates exerted a synergic effect favorable for beta-glucanase production by T. harzianum (4.25 U mL(-1)). (C) 2010 Elsevier B.V. All rights reserved. (AU)