Activity of Matrix Metalloproteinases in Bovine versus Human Dentine

Autor(es)

Kato, M. T. ^[1] ; Hannas, A. R. ^[1] ; Leite, A. L. ^[1] ; Bolanho, A. ^[1] ; Zarella, B. L. ^[1] ; Santos, J. ^[2] ; Carrilho, M. ^[3] ; Tjaderhane, L. ^{[4, 5]} ; Buzalaf, M. A. R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, BR-17012901 Bauru, SP - Brazil

^[2] Univ Estadual Campinas, Piracicaba Dent Sch, Endodont Area, Dept Restorat Dent, Piracicaba - Brazil

^[3] Univ Bandeirante Sao Paulo, Sch Dent, GEO UNIBAN, Sao Paulo - Brazil

^[4] Univ Oulu, Inst Dent, Oulu - Finland

^[5] Oulu Univ Hosp, Oulu - Finland

• Tipo de documento: Artigo Científico
• Fonte: Caries Research; v. 45, n. 5, p. 429-434, 2011.
• Citações Web of Science: 12

Resumo

Metalloproteinases (MMPs) have been implicated with metabolism of collagen in physiological and pathological processes in human dentine. As bovine teeth have been used as a substitute for human teeth in laboratory analysis, this study evaluated the activity of MMP-2 and -9 in bovine versus human dentine. Bovine and human dentine fragments, from crowns and roots, were powderized. Protein extraction was performed by two protocols: a neutral extraction with guanidine-HCl/EDTA (pH 7.4) and an acidic extraction with citric acid (pH 2.3). Gelatinolytic activities of extracts were revealed by zymography. MMP-2 and -9 were detected in crown and root dentine from bovine and human teeth. Total activities of MMP-2 were 11.4 +/- 2.2, 14.6 +/- 2.0, 9.7 +/- 1.2 and 12.4 +/- 0.9 ng/ml for bovine root, human root, bovine crown and human crown dentine, respectively. Corresponding activities for MMP-9 were 14.9 +/- 2.0, 15.3 +/- 1.3, 15.4 +/- 1.3 and 15.5 +/- 1.3 ng/ml, respectively. Bovine dentine was found to be a reliable substrate for studies involving the activity of MMP-2 and -9. Copyright (C) 2011 S. Karger AG, Basel (AU)

• Processo FAPESP: 07/04209-0 - Avaliação do efeito do chá verde, inibidor natural das metaloproteinases de matriz, sobre a erosão da dentina associada ou não à abrasão: um estudo in situ
• Beneficiário: Melissa Thiemi Kato
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 07/54618-4 - Avaliação da atividade de colagenases na degradação da dentina humana tratada por procedimentos restauradores adesivos
• Beneficiário: Marcela Rocha de Oliveira Carrilho
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 07/08389-3 - Avaliação do efeito de inibidores de metaloproteinases de matriz sobre a erosão dentária: estudos in situ / ex vivo
• Beneficiário: Marília Afonso Rabelo Buzalaf
• Modalidade de apoio: Auxílio à Pesquisa - Regular