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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Activity of Matrix Metalloproteinases in Bovine versus Human Dentine

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Author(s):
Kato, M. T. [1] ; Hannas, A. R. [1] ; Leite, A. L. [1] ; Bolanho, A. [1] ; Zarella, B. L. [1] ; Santos, J. [2] ; Carrilho, M. [3] ; Tjaderhane, L. [4, 5] ; Buzalaf, M. A. R. [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, BR-17012901 Bauru, SP - Brazil
[2] Univ Estadual Campinas, Piracicaba Dent Sch, Endodont Area, Dept Restorat Dent, Piracicaba - Brazil
[3] Univ Bandeirante Sao Paulo, Sch Dent, GEO UNIBAN, Sao Paulo - Brazil
[4] Univ Oulu, Inst Dent, Oulu - Finland
[5] Oulu Univ Hosp, Oulu - Finland
Total Affiliations: 5
Document type: Journal article
Source: Caries Research; v. 45, n. 5, p. 429-434, 2011.
Web of Science Citations: 12
Abstract

Metalloproteinases (MMPs) have been implicated with metabolism of collagen in physiological and pathological processes in human dentine. As bovine teeth have been used as a substitute for human teeth in laboratory analysis, this study evaluated the activity of MMP-2 and -9 in bovine versus human dentine. Bovine and human dentine fragments, from crowns and roots, were powderized. Protein extraction was performed by two protocols: a neutral extraction with guanidine-HCl/EDTA (pH 7.4) and an acidic extraction with citric acid (pH 2.3). Gelatinolytic activities of extracts were revealed by zymography. MMP-2 and -9 were detected in crown and root dentine from bovine and human teeth. Total activities of MMP-2 were 11.4 +/- 2.2, 14.6 +/- 2.0, 9.7 +/- 1.2 and 12.4 +/- 0.9 ng/ml for bovine root, human root, bovine crown and human crown dentine, respectively. Corresponding activities for MMP-9 were 14.9 +/- 2.0, 15.3 +/- 1.3, 15.4 +/- 1.3 and 15.5 +/- 1.3 ng/ml, respectively. Bovine dentine was found to be a reliable substrate for studies involving the activity of MMP-2 and -9. Copyright (C) 2011 S. Karger AG, Basel (AU)

FAPESP's process: 07/08389-3 - Evaluation of the effect of matrix metalloproteinases inhibitors on dental erosion: in situ / ex vivo studies
Grantee:Marília Afonso Rabelo Buzalaf
Support Opportunities: Regular Research Grants