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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Evaluation of the Frequency of Micronuclei in Exfoliated Cells from Oral Lesions Previously Identified by Toluidine Blue

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Autor(es):
de Oliveira, Pollyanna Francielli [1] ; Andrade, Augusto Faria [1] ; Malheiros, Fernanda Ferreira [1] ; de Lacerda, Suzie Aparecida [2] ; Campos, Alessandra Aparecida [1] ; Zaia, Jose Eduardo [1] ; Cecchi, Andrea de Oliveira [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Franca, BR-14404600 Franca, SP - Brazil
[2] Univ Sao Paulo, Fac Odontol Ribeirao Preto, BR-14049 Ribeirao Preto - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: ACTA CYTOLOGICA; v. 55, n. 4, p. 344-349, 2011.
Citações Web of Science: 3
Resumo

Objective: Patients using a removable prosthesis are susceptible to a variety of oral lesions that may progress to cancer. Toluidine blue (TB) staining is used to identify premalignant lesions, but the results are still controversial. Since micronuclei (MN) are a biomarker of genetic instability, the objective of this study was to determine the frequency of MN in white lesions of the oral mucosa and to compare the results with those of the TB test. Study Design: The study included 20 removable prosthesis users with white lesions that were previously classified as toluidine positive or negative. The frequency of MN was evaluated in exfoliated cells from lesions and normal mucosa. Nuclear anomalies were also registered. Results: A significant increase (p < 0.05) in the frequency of MN was observed in exfoliated cells from lesions compared to normal mucosal cells, and no relationship was seen with TB staining. Lifestyle factors or gender did not influence the results. Conclusions: The frequency of MN is a sensitive biomarker and can be used to predict genomic instability in white oral lesions. The MN assay may serve as a good parameter in the battery of tests used to identify high-risk individuals, contributing to the identification of the biological conditions of oral lesions. Copyright (C) 2011 S. Karger AG, Basel (AU)

Processo FAPESP: 09/06174-5 - Avaliação da frequência de micronúcleo em células esfoliadas de lesões orais
Beneficiário:Pollyanna Francielli de Oliveira
Modalidade de apoio: Bolsas no Brasil - Iniciação Científica