HPLC-FLD simultaneous determination of 13 polycyclic aromatic hydrocarbons: validation of an analytical procedure for soybean oils

Autor(es)

Camargo, Monica C. R. ^[1] ; Antoniolli, Paula R. ^[2] ; Vicente, Eduardo ^[1]

Afiliação do(s) autor(es)

^[1] Inst Tecnol Alimentos, Ctr Ciencia & Qualidade Alimentos, BR-13070178 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Inst Quim, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of the Brazilian Chemical Society; v. 22, n. 7, p. 1354-1361, 2011.
• Citações Web of Science: 13

Resumo

A method using high performance liquid chromatography with fluorescence detection (HPLC-FLD) was developed for the simultaneous determination of 13 polycyclic aromatic hydrocarbons (PAHs) in soybean oils. The sample preparation includes liquid-liquid extraction with dimethylformamide-water (9:1, v/v) followed by a clean-up and concentration step with C-18 SPE cartridges. The method showed good recoveries for most of the PAHs studied with values between 71 and 115%. The calibration curves were linear over the range tested (R² > 0.999). Detection and quantification limits varied between 0.02-0.76 µg kg-1 and 0.03-0.96 µg kg-1, respectively. The analytical procedure was successfully applied to evaluate the contamination of most consumed brands of soybean oils commercially available in the Brazilian market. The presence of PAHs was confirmed in all samples analyzed with mean values ranging from 0.20 to 5.93 µg kg-1. (AU)

• Processo FAPESP: 05/59974-8 - Contaminação de alimentos por hidrocarbonetos policíclicos aromáticos (HPAs)
• Beneficiário: Mônica Cristiane Rojo de Camargo
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores