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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

HPLC-FLD simultaneous determination of 13 polycyclic aromatic hydrocarbons: validation of an analytical procedure for soybean oils

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Author(s):
Camargo, Monica C. R. [1] ; Antoniolli, Paula R. [2] ; Vicente, Eduardo [1]
Total Authors: 3
Affiliation:
[1] Inst Tecnol Alimentos, Ctr Ciencia & Qualidade Alimentos, BR-13070178 Campinas, SP - Brazil
[2] Univ Estadual Campinas, Inst Quim, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of the Brazilian Chemical Society; v. 22, n. 7, p. 1354-1361, 2011.
Web of Science Citations: 13
Abstract

A method using high performance liquid chromatography with fluorescence detection (HPLC-FLD) was developed for the simultaneous determination of 13 polycyclic aromatic hydrocarbons (PAHs) in soybean oils. The sample preparation includes liquid-liquid extraction with dimethylformamide-water (9:1, v/v) followed by a clean-up and concentration step with C-18 SPE cartridges. The method showed good recoveries for most of the PAHs studied with values between 71 and 115%. The calibration curves were linear over the range tested (R² > 0.999). Detection and quantification limits varied between 0.02-0.76 µg kg-1 and 0.03-0.96 µg kg-1, respectively. The analytical procedure was successfully applied to evaluate the contamination of most consumed brands of soybean oils commercially available in the Brazilian market. The presence of PAHs was confirmed in all samples analyzed with mean values ranging from 0.20 to 5.93 µg kg-1. (AU)