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Development of novel methodology for the routine monitoring of monohydroxy metabolites of polycyclic aromatic hydrocarbons in numerous urine samples

Abstract

Reducing the sample temperature offers several advantages to molecular luminescence (fluorescence and/or phosphorescence) spectroscopy. Analysis at cryogenic temperatures often improves spectral resolution, increases emission quantum yields and minimizes - or even eliminates - complications with oxygen quenching and energy transfer. Although spectral resolution improvement is almost always observed with lowering the temperature to 77 K or below, temperature effects on spectral narrowing are particularly noticeable in Shpol'skii spectroscopy. This technique combines the inherent sensitivity of fluorescence and phosphorescence with the selectivity of vibrationally resolved spectra. Unfortunately, the Shpol'skii phenomenon requires a specific guest-host combination that has confined Shpol'skii spectroscopy to the analysis of polycyclic aromatic hydrocarbons (PAHs) that are compatible with non-polar matrixes (n-alkanes). PAHs are important environmental pollutants of great environmental and toxicological importance.The end goal of this proposition is to generalize the methods of Shpol'skii spectroscopy to a variety of polar compounds by using polar solvents. Initial work is proposed here to investigate the Shpol'skii behavior of monohydroxy metabolites of PAHs (OH-PAHs) in frozen organic alcohols, provide a more general model for Shpol'skii spectroscopy and allow more widespread use of the methodology. Due to the short average elimination lifetime from the body, the quantitative determination of OH-PAHs in urine samples provides accurate information on recent environmental exposure to PAHs. Although chromatographic techniques provide adequate limits of detection for OH-PAHs in urine samples, their experimental procedures are time consuming and expensive. The research presented here will provide easy-to-use and cost-effective methodology well-suited methodology for data collection from statistically meaningful population sizes. (AU)

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MATHEUS GALLIMBERTI; BRUNO A. ROCHA; VANESSA C. O. SOUZA; ANDRES D. CAMPIGLIA; FERNANDO BARBOSA JR. Determination of Bisphenol A in Paper Products by Synchronous Fluorescence Spectroscopy and Estimation of Daily Exposure. Journal of the Brazilian Chemical Society, v. 31, n. 10, p. 2146-2154, Out. 2020. Web of Science Citations: 0.
ALFARHANI, BASSAM; AL-TAMEEMI, MAHA; SCHENONE, AGUSTINA V.; GOICOECHEA, HECTOR C.; BARBOSA, JR., FERNANDO; CAMPIGLIA, ANDRES D. Room temperature fluorescence spectroscopy of benzo[a]pyrene metabolites on octadecyl extraction membranes. Microchemical Journal, v. 129, p. 83-89, NOV 2016. Web of Science Citations: 1.
MOORE, ANTHONY F. T.; GOICOECHEA, HECTOR C.; BARBOSA, JR., FERNANDO; CAMPIGLIA, ANDRES D. Parallel Factor Analysis of 4.2 K Excitation-Emission Matrices for the Direct Determination of Dibenzopyrene Isomers in Coal-Tar Samples with a Cryogenic Fiber-Optic Probe Coupled to a Commercial Spectrofluorimeter. Analytical Chemistry, v. 87, n. 10, p. 5232-5239, MAY 19 2015. Web of Science Citations: 8.
BORGES, ENDLER MARCEL; VOLMER, DIETRICH A.; GALLIMBERTI, MATHEUS; DE SOUZA, DEISE FERREIRA; DE SOUZA, EDSON LUIZ; BARBOSA, JR., FERNANDO. Evaluation of macro- and microelement levels for verifying the authenticity of organic eggs by using chemometric techniques. ANALYTICAL METHODS, v. 7, n. 6, p. 2577-2584, 2015. Web of Science Citations: 6.

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