Use of fluorescence spectroscopy in the determination of contamination and oxidative processes in edible vegetable oils

• Grant number: 18/20761-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2019
• End date: April 30, 2023
• Field of knowledge: Interdisciplinary Subjects
• Principal Investigator: Lilia Coronato Courrol
• Grantee: Carla Regina Borges Lopes
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil

Abstract

The world consumption of vegetable oils in food products was over 104 million tons in the last year. Edible vegetable oils have a high incidence of contamination by Polycyclic Aromatic Hydrocarbons (PAHs) and mycotoxins, indicated as possible responsible for the development of neoplasias. In addition, they are highly susceptible to lipid oxidation, complex reactions that result in the production of toxic substances, with serious nutritional consequences to their consumers. In this context, strict quality control of these products is of utmost importance, with the continuous improvement of the analytical techniques used and the development of new and efficient methods capable of complementing or replacing them. Considering this scenario, the present research project proposes the use of fluorescence spectroscopy for the detection of contaminants (PAHs, microorganisms and aflatoxins) and determination of lipid oxidation status. The proposed method consists in the extensive study of the fluorescence spectra of vegetable oils, relating changes in emission intensity and wavelength with the presence of these contaminants and with the advancement of oxidation reactions, in addition to determining the generation of singlet oxygen and its importance in oxidative processes. The advantages of the fluorescence-based methods are the high sensitivity, fast results and the possibility of direct analysis of the sample, avoiding expensive preparation procedures, and portable equipment for in situ analysis. The fulfillment of the proposed objectives will result in a method capable of determining quality indicators of vegetable oils, being able to be applied in the food industry and institutions responsible for food quality control. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BORGES LOPES, CARLA REGINA; TORRES DA SILVA, HERON DOMINGUEZ; COURROL, LILIA CORONATO; IEEE. Fluorescence spectroscopy study of conventional and organic soybean oil heated to 270 degrees C. 2021 SBFOTON INTERNATIONAL OPTICS AND PHOTONICS CONFERENCE (SBFOTON IOPC), v. N/A, p. 4-pg., 2021-01-01. (18/20761-0, 17/23686-6)

BORGES LOPES, CARLA REGINA; COURROL, LILIA CORONATO; IEEE. Effect of the addition of thermoxidized soybean oil on the fluorescence spectra of silver nanoparticles synthesized with extract of Mimusops coriacea. 2022 SBFOTON INTERNATIONAL OPTICS AND PHOTONICS CONFERENCE (SBFOTON IOPC), v. N/A, p. 4-pg., 2022-01-01. (18/20761-0, 17/23686-6)

BORGES LOPES, CARLA REGINA; TORRES DA SILVA, HERON DOMINGUEZ; COURROL, LILIA CORONATO; IEEE. Comparative spectroscopic studies between conventional and organic soybean oils. 2021 SBFOTON INTERNATIONAL OPTICS AND PHOTONICS CONFERENCE (SBFOTON IOPC), v. N/A, p. 4-pg., 2021-01-01. (18/20761-0, 17/23686-6)

BORGES LOPES, CARLA REGINA; COURROL, LILIA CORONATO; IEEE. Chlorophyll Fluorescence Analysis to Evaluate the Photo-oxidation Process in Organic Soybean Oil. 2023 INTERNATIONAL CONFERENCE ON OPTICAL MEMS AND NANOPHOTONICS, OMN AND SBFOTON INTERNATIONAL OPTICS AND PHOTONICS CONFERENCE, SBFOTON IOPC, v. N/A, p. 2-pg., 2023-01-01. (18/20761-0)

LOPES, ISABELA SANTOS; COURROL, LILIA CORONATO; IEEE. Evaluation of silver nanoparticles synthesized with ALA and plant extract in seed nanopriming. 2023 INTERNATIONAL CONFERENCE ON OPTICAL MEMS AND NANOPHOTONICS, OMN AND SBFOTON INTERNATIONAL OPTICS AND PHOTONICS CONFERENCE, SBFOTON IOPC, v. N/A, p. 2-pg., 2023-01-01. (18/20761-0)

LOPES, CARLA REGINA BORGES; COURROL, LILIA CORONATO. Evaluation of Steady-State and Time-Resolved Fluorescence Spectroscopy as a Method for Assessing the Impact of Photo-Oxidation on Refined Soybean Oils. FOODS, v. 12, n. 9, p. 16-pg., 2023-04-30. (18/20761-0)

LOPES, ISABELA SANTOS; FRANZOLIN, MARCIA REGINA; BARRETO, SUSANA DE SOUZA; BORGES LOPES, CARLA REGINA; COURROL, LILIA CORONATO; IEEE. Aminolevulinic acid-based metallic nanoparticles: Applications in Agriculture. 2022 SBFOTON INTERNATIONAL OPTICS AND PHOTONICS CONFERENCE (SBFOTON IOPC), v. N/A, p. 5-pg., 2022-01-01. (18/20761-0)