Antimicrobial resistance and prevalence of resistance genes in intestinal Bacteroidales strains

Autor(es)

Nakano, Viviane ^[1] ; do Nascimento e Silva, Amanda ^[1] ; Castillo Merino, Victor Rafael ^[1] ; Wexler, Hannah M. ^{[2, 3]} ; Avila-Campos, Mario Julio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Anaerobe Lab, Sao Paulo - Brazil

^[2] Greater Los Angeles Vet Adm Healthcare Syst, Los Angeles, CA - USA

^[3] Univ Calif Los Angeles, Dept Med, Los Angeles, CA 90024 - USA

• Tipo de documento: Artigo Científico
• Fonte: Clinics; v. 66, n. 4, p. 543-547, 2011.
• Citações Web of Science: 36

Resumo

OBJECTIVE: This study examined the antimicrobial resistance profile and the prevalence of resistance genes in Bacteroides spp. and Parabacteroides distasonis strains isolated from children's intestinal microbiota. METHODS: The susceptibility of these bacteria to 10 antimicrobials was determined using an agar dilution method. β-lactamase activity was assessed by hydrolysis of the chromogenic cephalosporin of 114 Bacteriodales strains isolated from the fecal samples of 39 children, and the presence of resistance genes was tested using a PCR assay. RESULTS: All strains were susceptible to imipenem and metronidazole. The following resistance rates were observed: amoxicillin (93%), amoxicillin/clavulanic acid (47.3%), ampicillin (96.4%), cephalexin (99%), cefoxitin (23%), penicillin (99%), clindamycin (34.2%) and tetracycline (53.5%). P-lactamase production was verified in 92% of the evaluated strains. The presence of the cfiA, cepA, ermF, tetQ and nim genes was observed in 62.3%, 76.3%, 27%, 79.8% and 7.8% of the strains, respectively. CONCLUSIONS: Our results indicate an increase in the resistance to several antibiotics in intestinal Bacteroides spp. and Parabacteroides distasonis and demonstrate that these microorganisms harbor antimicrobial resistance genes that may be transferred to other susceptible intestinal strains. (AU)

• Processo FAPESP: 09/03792-0 - Expressão dos genes bmeABC de bombas de efluxo de Bacteroides fragilis em Escherichia coli, caracterização do perfil de substrato das bombas bmeABC e sua relação com a resistência aos antimicrobianos
• Beneficiário: Viviane Nakano
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores
• Processo FAPESP: 08/57330-4 - Expressão dos genes bmeABC de bombas de efluxo de Bacteroides fragilis em Escherichia coli, caracterização do perfil de substrato das bombas bmeABC e sua relação com a resistência aos antimicrobianos
• Beneficiário: Viviane Nakano
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores