Effects of eccentric and concentric training on capillarization and myosin heavy chain contents in rat skeletal muscles after hindlimb suspension

Autor(es)

Cornachione, Anabelle ^[1] ; Cacao-Benedini, Leticia O. ^[1] ; Martinez, Edson Z. ^[2] ; Neder, Luciano ^[3] ; Mattiello-Sverzut, Ana Claudia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Biomech Med & Rehabil Locomotor Apparat, Ribeirao Preto Sch Med, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Dept Social Med, Ribeirao Preto Sch Med, BR-14049900 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Dept Pathol, Ribeirao Preto Sch Med, BR-14049900 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ACTA HISTOCHEMICA; v. 113, n. 3, p. 277-282, 2011.
• Citações Web of Science: 18

Resumo

We studied the effects of different protocols of post-disuse rehabilitation on angiogenesis and myosin heavy chain (MHC) content in rat hindlimb muscles after caudal suspension. Thirty female Wistar rats were divided into five groups: (1) Control I, (2) Control II, (3) Suspended, (4) Suspended trained on declined treadmill, and (5) Suspended trained on flat treadmill. Fragments of the soleus and tibialis anterior (TA) muscles were frozen and processed by electrophoresis and immunohistochemistry (CD31 antibody). Hindlimb suspension caused reduction of capillary/fiber (C/F) ratios and contents of MHC type I (MHCI) in the soleus in parallel to increased capillary density. Flat treadmill protocols increased the content of the MHCI isoform. The C/F ratio was increased by concentric training after hypokinesis, but was not modified by eccentric training, which caused a greater reduction of capillary density compared to the other protocols. In the TA muscle, hindlimb suspension caused a non-significant increase in capillary density and C/F ratio with limited changes in MHC. The present data demonstrate that the different training protocols adopted and the functional performance of the muscles analyzed caused specific changes in capillarization and in the content of the various MHC types. (C) 2010 Published by Elsevier GmbH. (AU)

• Processo FAPESP: 04/10496-4 - Efeitos morfológicos da atividade em esteira nos músculos sóleo e tibial anterior de ratas após suspensão dos membros posteriores pela cauda
• Beneficiário: Anabelle Silva Cornachione
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 04/10495-8 - Efeitos morfológicos da atividade em esteira nos músculos sóleo e tibial anterior de ratas após suspensão dos membros posteriores pela cauda
• Beneficiário: Ana Cláudia Mattiello-Sverzut
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 05/51524-3 - Efeitos morfológicos da atividade em esteira nos músculos sóleo e tibial anterior de ratas após suspensão dos membros posteriores pela cauda
• Beneficiário: Letícia Cação Benedini de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica