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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of eccentric and concentric training on capillarization and myosin heavy chain contents in rat skeletal muscles after hindlimb suspension

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Author(s):
Cornachione, Anabelle [1] ; Cacao-Benedini, Leticia O. [1] ; Martinez, Edson Z. [2] ; Neder, Luciano [3] ; Mattiello-Sverzut, Ana Claudia [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Dept Biomech Med & Rehabil Locomotor Apparat, Ribeirao Preto Sch Med, BR-14049900 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Dept Social Med, Ribeirao Preto Sch Med, BR-14049900 Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Dept Pathol, Ribeirao Preto Sch Med, BR-14049900 Ribeirao Preto, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: ACTA HISTOCHEMICA; v. 113, n. 3, p. 277-282, 2011.
Web of Science Citations: 18
Abstract

We studied the effects of different protocols of post-disuse rehabilitation on angiogenesis and myosin heavy chain (MHC) content in rat hindlimb muscles after caudal suspension. Thirty female Wistar rats were divided into five groups: (1) Control I, (2) Control II, (3) Suspended, (4) Suspended trained on declined treadmill, and (5) Suspended trained on flat treadmill. Fragments of the soleus and tibialis anterior (TA) muscles were frozen and processed by electrophoresis and immunohistochemistry (CD31 antibody). Hindlimb suspension caused reduction of capillary/fiber (C/F) ratios and contents of MHC type I (MHCI) in the soleus in parallel to increased capillary density. Flat treadmill protocols increased the content of the MHCI isoform. The C/F ratio was increased by concentric training after hypokinesis, but was not modified by eccentric training, which caused a greater reduction of capillary density compared to the other protocols. In the TA muscle, hindlimb suspension caused a non-significant increase in capillary density and C/F ratio with limited changes in MHC. The present data demonstrate that the different training protocols adopted and the functional performance of the muscles analyzed caused specific changes in capillarization and in the content of the various MHC types. (C) 2010 Published by Elsevier GmbH. (AU)