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Influence of aerobic or resistance physical exercise on skeletal muscle of rats with myocardial infarction

Grant number: 14/23592-3
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): April 01, 2016
Effective date (End): March 31, 2019
Field of knowledge:Health Sciences - Medicine
Principal Investigator:Marina Politi Okoshi
Grantee:Mariana Janini Gomes
Home Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
Associated scholarship(s):16/15586-9 - NOX2 and membrane repair proteins in unloading-induced muscle atrophy, BE.EP.DR

Abstract

Oxidative stress and imbalance between anabolic and catabolic processes are involved in heart failure-associated skeletal muscle changes. Several positive effects from aerobic exercise have been described in heart failure. However, despite recent studies reporting beneficial effects from resistive exercise, the mechanisms involved in its effects are not completely clear. Resistive exercise increases muscle trophicity by activating satellite cells. We have not identified studies evaluating the effects of resistive exercise on skeletal muscle during cardiac remodeling and heart failure. The purpose of this study is to compare the effects of aerobic and resistive exercise started during compensated cardiac remodeling on phenotypical and molecular changes that occur during myocardial infarction (MI)-induced heart failure. Three months after inducing MI, Wistar rats will be assigned into four groups: Sham; sedentary MI; MI subjected to aerobic exercise; and MI subjected to resistive exercise. The rats will be trained three times a week for three months on a treadmill running or resistance exercise on stairs. Echocardiogram will be performed before and after training. The percentage of infarcted area and muscle throphicity will be determined by morphometric analysis. Protein expression of MAPKs, NF-kappaB, and ubiquitin-proteasome pathways, TNF-alfa and Pax-7 will be analyzed by Western-blot. Total ROS generation will be assessed by quantifying products derived from oxidation of dihidroetidio by HPLC. Determination of energy metabolism and the antioxidant enzymes catalase, superoxide dismutase, and glutathione peroxidase will be quantified by spectrophotometry; satellite cells activation will be evaluated by immunofluorescence for MyoD, NCAM, and neonatal isoform of myosin heavy chains. Serum concentration of TNF-alfa will be assessed by ELISA. Statistical analysis: ANOVA

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MARIANA JANINI GOMES; LUANA URBANO PAGAN; MARINA POLITI OKOSHI. Non-Pharmacological Treatment of Cardiovascular Disease | Importance of Physical Exercise. Arquivos Brasileiros de Cardiologia, v. 113, n. 1, p. 9-10, Jul. 2019.
GOMES, MARIANA JANINI; MARTINEZ, PAULA FELIPPE; PAGAN, LUANA URBANO; DAMATTO, RICARDO LUIZ; MARIANO CEZAR, MARCELO DIACARDIA; RUIZ LIMA, ALINE REGINA; OKOSHI, KATASHI; OKOSHI, MARINA POLITI. Skeletal muscle aging: influence of oxidative stress and physical exercise. ONCOTARGET, v. 8, n. 12, p. 20428-20440, 2017. Web of Science Citations: 35.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.