Behavior of Wild-type and Transfected S2 Cells Cultured in Two Different Media

Autor(es)

Batista, Fabiana R. X. ^[1] ; Greco, Katia N. ^[2] ; Astray, Renato M. ^[2] ; Jorge, Soraia A. C. ^[2] ; Augusto, Elisabeth F. P. ^[3] ; Pereira, Carlos A. ^[2] ; Mendonca, Ronaldo Z. ^[2] ; Moraes, Angela M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Fac Engn Quim, Dept Proc Biotecnol, BR-13083852 Campinas, SP - Brazil

^[2] Inst Butantan, Lab Imunol Viral, BR-05503900 Sao Paulo - Brazil

^[3] Inst Pesquisas Tecnol Estado Sao Paulo SA, Lab Biotecnol Ind, BR-05508901 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Applied Biochemistry and Biotechnology; v. 163, n. 1, p. 1-13, JAN 2011.
• Citações Web of Science: 5

Resumo

An animal protein-free medium composed of IPL-41 containing 6 g L(-1) yeastolate ultrafiltrate, 10 g L(-1) glucose, 2 g L(-1) lactose, 5 g L(-1) glutamine, 1% lipid emulsion, and 0.1% Pluronic F-68 was used for producing recombinant proteins in batch mode employing two cell lines, S2AcRVGP2k expressing the G glycoprotein from rabies virus (RVGP) and S2AcHBsAgHy-9C expressing the surface antigen of hepatitis B virus (HBsAg), both obtained from Drosophila melanogaster S2 cells. Growth of wild-type S2 cells was also evaluated in the same medium. Cell behavior in the tested medium was compared to that verified in Sf900 IIA (R). The results show that in shake flasks, S2AcRVGP2k and S2AcHBsAgHy-9C cells reached around 2 x 10(7) cells mL(-1) in both media. In supplemented IPL-41 and Sf900 IIA (R) media, S2AcRVGP2k cells produced 367 ng RVGP mL(-1) and 638 ng RVGP mL(-1), respectively, while S2AcHBsAgHy-9C cells correspondently produced 573 ng HBsAg mL(-1) and 322 ng HBsAg mL(-1) in the mentioned media. In stirred tanks, S2AcRVGP2k cells reached 3 x 10(7) cells mL(-1) and produced up to 758 ng RVGP mL(-1). In general, glucose was consumed by cells, while lactate and ammonia were produced. (AU)

• Processo FAPESP: 02/09482-3 - Expressão de genes heterólogos em células de dípteros: biologia molecular e engenharia de processos
• Beneficiário: Carlos Augusto Pereira
• Modalidade de apoio: Auxílio à Pesquisa - Temático