In situ hybridization detection of homeobox genes reveals distinct expression patterns in oral squamous cell carcinomas

Autor(es)

Liborio, Tatiana N. ^[1] ; Acquafreda, Thais ; Matizonkas-Antonio, Luciana F. ; Silva-Valenzuela, Maria G. ^[2] ; Ferraz, Alberto R. ^[3] ; Nunes, Fabio D. ^[4]

Afiliação do(s) autor(es)

^[1] Univ Fed Amazonas, Sch Med, Dept Pathol, Manaus, Amazonas - Brazil

^[2] Univ Sao Paulo, Dept Biochem, Inst Chem, BR-05508000 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Head & Neck Surg Serv, Hosp Clin, BR-05508000 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Fac Odontol, Dept Oral Pathol, Sch Dent, BR-05508000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Histopathology; v. 58, n. 2, p. 225-233, JAN 2011.
• Citações Web of Science: 12

Resumo

Aims: To analyse the expression of three homeobox genes (HOXA7, PITX1 and PRRX1) in oral squanous cell carcinomas (OSCC) and the relationship of such expression to certain distinct histopathological features of OSCC and in comparison to adjacent non-neoplastic epithelium (NT). Methods and results: Digoxigenin-labelled riboprobes that are specific for each homeobox gene were generated and in situ hybridization was carried out on frozen sections. In NT samples, HOXA7 and PITX1 transcripts were found more frequently in all epithelial layers, while PRRX1 was expressed in the basal layer. With OSCC samples, expression of the three genes was associated with all histological features. However, the HOXA7 and PITX1 signals were more intense in sheets and nests and PRRX1 in small nests and isolated cells. Conclusion: HOXA7, PIXT1 and PRRX1 homeobox genes have different patterns of expression in OSCC depending on its histological features. (AU)

• Processo FAPESP: 01/13644-6 - Estudo da expressão de genes homeobox em carcinomas epidermoides de boca
• Beneficiário: Fabio Daumas Nunes
• Modalidade de apoio: Auxílio à Pesquisa - Regular