Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In situ hybridization detection of homeobox genes reveals distinct expression patterns in oral squamous cell carcinomas

Full text
Author(s):
Liborio, Tatiana N. [1] ; Acquafreda, Thais ; Matizonkas-Antonio, Luciana F. ; Silva-Valenzuela, Maria G. [2] ; Ferraz, Alberto R. [3] ; Nunes, Fabio D. [4]
Total Authors: 6
Affiliation:
[1] Univ Fed Amazonas, Sch Med, Dept Pathol, Manaus, Amazonas - Brazil
[2] Univ Sao Paulo, Dept Biochem, Inst Chem, BR-05508000 Sao Paulo - Brazil
[3] Univ Sao Paulo, Head & Neck Surg Serv, Hosp Clin, BR-05508000 Sao Paulo - Brazil
[4] Univ Sao Paulo, Fac Odontol, Dept Oral Pathol, Sch Dent, BR-05508000 Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Histopathology; v. 58, n. 2, p. 225-233, JAN 2011.
Web of Science Citations: 12
Abstract

Aims: To analyse the expression of three homeobox genes (HOXA7, PITX1 and PRRX1) in oral squanous cell carcinomas (OSCC) and the relationship of such expression to certain distinct histopathological features of OSCC and in comparison to adjacent non-neoplastic epithelium (NT). Methods and results: Digoxigenin-labelled riboprobes that are specific for each homeobox gene were generated and in situ hybridization was carried out on frozen sections. In NT samples, HOXA7 and PITX1 transcripts were found more frequently in all epithelial layers, while PRRX1 was expressed in the basal layer. With OSCC samples, expression of the three genes was associated with all histological features. However, the HOXA7 and PITX1 signals were more intense in sheets and nests and PRRX1 in small nests and isolated cells. Conclusion: HOXA7, PIXT1 and PRRX1 homeobox genes have different patterns of expression in OSCC depending on its histological features. (AU)