Phosphoproteomics Profiling Suggests a Role for Nuclear beta IPKC in Transcription Processes of Undifferentiated Murine Embryonic Stem Cells

Autor(es):Mostrar menos -

Costa-Junior, Hello Miranda ^[1] ; Garavello, Nicole Milare ^[2] ; Duarte, Mariana Lemos ^[2] ; Berti, Denise Aparecida ^[2] ; Glaser, Talita ^[2] ; de Andrade, Alexander ^[3] ; Labate, Carlos A. ^[3] ; da Silva Ferreira, Andre Teixeira ^[4] ; Aguilar Perales, Jonas Enrique ^[4] ; Xavier-Neto, Jose ^[1] ; Krieger, Jose Eduardo ^[1] ; Schechtman, Deborah ^[2]

Afiliação do(s) autor(es)

^[1] Inst Coracao, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Dept Genet, ESALQ, BR-05508 Sao Paulo - Brazil

^[4] Inst Oswaldo Cruz, BR-20001 Rio De Janeiro - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PROTEOME RESEARCH; v. 9, n. 12, p. 6191-6206, DEC 2010.
• Citações Web of Science: 7

Resumo

Protein kinase C (PKC) plays a key role in embryonic stem cell (ESC) proliferation, self-renewal and differentiation However, the function of specific PKC Isoenzymes have yet to be determined Of the PKCs expressed in undifferentiated ESCs, beta IPKC was the only isoenzyme abundantly expressed in the nuclei To investigate the role of beta IPKC in these cells, we employed a phosphoproteomics strategy and used two classical (cPKC) peptide modulators and one beta IPKC-specific inhibitor peptide We identified 13 nuclear proteins that are direct or indirect beta IPKC substrates in undifferentiated ESCs These proteins are known to be involved in regulating transcription, splicing, and chromatin remodeling during proliferation and differentiation Inhibiting beta IPKC had no effect on DNA synthesis in undifferentiated ESCs However, upon differentiation many cells seized to express beta IPKC and beta IPKC was frequently found in the cytoplasm Taken together, our results suggest that beta IPKC takes part in the processes that maintain ESCs in their undifferentiated state (AU)

• Processo FAPESP: 05/54188-4 - Determinação do papel da proteína quinase C na diferenciação e proliferação
• Beneficiário: Deborah Schechtman
• Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores