| Texto completo | |
| Autor(es): |
Sabino-Silva, Robinson
[1]
;
Alves-Wagner, Ana B. T.
[1]
;
Burgi, Katia
[1]
;
Okamoto, Maristela M.
[1]
;
Alves, Adilson S.
[1]
;
Lima, Guilherme A.
[1]
;
Freitas, Helayne S.
[1]
;
Antunes, Vagner R.
[1]
;
Machado, Ubiratan F.
[1]
Número total de Autores: 9
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-05505900 Sao Paulo - Brazil
Número total de Afiliações: 1
|
| Tipo de documento: | Artigo Científico |
| Fonte: | AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM; v. 299, n. 6, p. E1028-E1037, DEC 2010. |
| Citações Web of Science: | 14 |
| Resumo | |
Salivary gland dysfunction is a feature in diabetes and hypertension. We hypothesized that sodium-glucose cotransporter 1 (SGLT1) participates in salivary dysfunctions through a sympathetic- and protein kinase A (PKA)-mediated pathway. In Wistar-Kyoto (WKY), diabetic WKY (WKY-D), spontaneously hypertensive (SHR), and diabetic SHR (SHR-D) rats, PKA/SGLT1 proteins were analyzed in parotid and submandibular glands, and the sympathetic nerve activity (SNA) to the glands was monitored. Basal SNA was threefold higher in SHR (P < 0.001 vs. WKY), and diabetes decreased this activity (similar to 50%, P < 0.05) in both WKY and SHR. The catalytic subunit of PKA and the plasma membrane SGLT1 content in acinar cells were regulated in parallel to the SNA. Electrical stimulation of the sympathetic branch to salivary glands increased (similar to 30%, P < 0.05) PKA and SGLT1 expression. Immunohistochemical analysis confirmed the observed regulations of SGLT1, revealing its location in basolateral membrane of acinar cells. Taken together, our results show highly coordinated regulation of sympathetic activity upon PKA activity and plasma membrane SGLT1 content in salivary glands. Furthermore, the present findings show that diabetic- and/or hypertensive-induced changes in the sympathetic activity correlate with changes in SGLT1 expression in basolateral membrane of acinar cells, which can participate in the salivary glands dysfunctions reported by patients with these pathologies. (AU) | |
| Processo FAPESP: | 07/50554-1 - Transportadores de glicose e Diabetes mellitus: contribuição ao conhecimento da regulação da glicemia e do desenvolvimento de complicações |
| Beneficiário: | Ubiratan Fabres Machado |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 06/60833-2 - Regulação da expressão gênica do co-transportador Na+/glicose (SGLT1) e do transportador de glicose (GLUT1) em glândulas parótidas e submandibulares de ratos hipertensos diabéticos |
| Beneficiário: | Robinson Sabino da Silva |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |