Clinical Investigation of the Efficacy of Chemomechanical Preparation with Rotary Nickel-Titanium Files for Removal of Endotoxin from Primarily Infected Root Canals

Autor(es)

Martinho, Frederico C. ^[1] ; Chiesa, Wanderson M. M. ^[1] ; Marinho, Ariane C. S. ^[1] ; Zaia, Alexandre A. ^[1] ; Ferraz, Caio C. R. ^[1] ; Almeida, Jose E. A. ^[1] ; Souza-Filho, Francisco J. ^[1] ; Gomes, Brenda P. F. A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, Endodont Div, Dept Restorat Dent, Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ENDODONTICS; v. 36, n. 11, p. 1766-1769, NOV 2010.
• Citações Web of Science: 31

Resumo

Introduction: This clinical study was conducted to investigate the ability of chemomechanical preparation with 25% NaOCl + 17% ethylenediaminetetraacetic acid (EDTA) and rotary nickel-titanium (NiTi) system in removing endotoxin from primary root canal infection with apical periodontitis. Methods: Twenty-one root canals with necrotic pulps were selected. Samples were collected before (s1) and after chemomechanical preparation (s2). The limulus amebocyte lysate (LAL) assay was used to quantify endotoxins. Results: The LAL assay indicated that endotoxins were present in 100% of the root canals investigated (19/19) before (s1) and after chemomechanical preparation (s2). Analyses of the quantitative data revealed that the endotoxin content was significantly reduced at s2 (98.06%) compared with that at Si (P < .05). Conclusions: Our findings indicate that chemomechanical preparation with 2.5% NaOCl + 17% EDTA and rotary NiTi files was effective in reducing endotoxin load in the root canal infection from primarily infected teeth with apical periodontitis. (1 Endod 2010;36:1766-1769) (AU)

• Processo FAPESP: 08/58299-3 - Estudo microbiológico e de endotoxinas em canais radiculares de dentes com necrose pulpar e lesão periapical e avaliação da atividade endotóxica de LPS isolados de canais radiculares infectados
• Beneficiário: Brenda Paula Figueiredo de Almeida Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/58518-4 - Análise da microbiota de canais radiculares de dentes humanos infectados através da cultura, clonagem e sequenciamento do gene 16S rRNA
• Beneficiário: Brenda Paula Figueiredo de Almeida Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/57954-8 - Estudo microbiológico, quantificação de endotoxinas e investigação da atividade endotóxica de diferentes LPS bacterianos isolados de canais radiculares de dentes com necrose pulpar e presença de lesão periapiacal
• Beneficiário: Frederico Canato Martinho
• Modalidade de apoio: Bolsas no Brasil - Doutorado