Treponema Species Detected in Infected Root Canals and Acute Apical Abscess Exudates

Autor(es)

Montagner, Francisco ^[1] ; Jacinto, Rogerio C. ^[2] ; Signoretti, Fernanda G. C. ; Gomes, Brenda P. F. A. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Fed Rio Grande do Sul, Endodont Div, Dept Conservat Dent, UFRGS, Porto Alegre, RS - Brazil

^[2] Univ Fed Pelotas, Endodont Div, Pelotas, RS - Brazil

^[3] Univ Estadual Campinas, Fac Odontol Piracicaba, Piracicaba Dent Sch, Dept Restorat Dent, Endodont Div, UNICAMP, BR-13414018 Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ENDODONTICS; v. 36, n. 11, p. 1796-1799, NOV 2010.
• Citações Web of Science: 15

Resumo

Introduction: Different microbial communities have been associated with acute endodontic infections. The majority of the microorganisms are as yet uncultivable or difficult to grow under current laboratory conditions. Treponema species are strict anaerobic bacteria that are involved in several oral diseases. The aim of this study was to detect the presence of Treponema species in infected root canals (RCs) and exudates related to acute apical abscesses (AAAs) as well as to determine positive association between targeted species and clinical signs/symptoms. Methods: Paired samples of infected RCs and AAAs were collected from 20 subjects. Nested polymerase chain reaction assay with species-specific primers for 165 rDNA and downstream intergenic spacer region was used for microbial detection. The frequency of species and statistical associations between species and signs/symptoms of endodontic origin as well as their simultaneous detection in both milieus were investigated. Results: The most frequently detected species were T. socranskii (RC, 17/20; AM, 15/20), T. denticola (RC, 8/20; AAA, 11/20); T. medium (RC, 6/20; AM, 9/20); and T. amylovorum (RC, 5/20; AM, 9/20). Positive correlation was found for simultaneous presence of I denticola in both RCs and AAAs (p = 0.01). Positive association was observed between I medium and T. vincentii (p = .037). No positive statistical association was observed between the targeted species and signs/symptoms. Conclusions: The high incidence of Treponema species in RC and AAA samples from the same tooth indicated that they are important pathogens in acute endodontic infections. (1 Endod 2010;36:1796-1799) (AU)

• Processo FAPESP: 08/56425-1 - Caracterização fenotipica e molecular da microbiota de dentes com necrose pulpar e abscessos periapicais e susceptibilidade antimicrobiana de bactérias anaeróbias estritas isoladas
• Beneficiário: Francisco Montagner
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 08/58299-3 - Estudo microbiológico e de endotoxinas em canais radiculares de dentes com necrose pulpar e lesão periapical e avaliação da atividade endotóxica de LPS isolados de canais radiculares infectados
• Beneficiário: Brenda Paula Figueiredo de Almeida Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/58518-4 - Análise da microbiota de canais radiculares de dentes humanos infectados através da cultura, clonagem e sequenciamento do gene 16S rRNA
• Beneficiário: Brenda Paula Figueiredo de Almeida Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/57551-0 - Microbiota dos canais radiculares de dentes com insucesso do tratamento endodôntico e análise da suscetibilidade antimicrobiana e dos fatores de virulência dos Enterococcus faecalis isolados
• Beneficiário: Brenda Paula Figueiredo de Almeida Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/06162-4 - Análise da microbiota de canais radiculares de dentes humanos infectados através da cultura, clonagem e sequenciamento do gene 16S rRNA
• Beneficiário: Frederico Canato Martinho
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico