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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Treponema Species Detected in Infected Root Canals and Acute Apical Abscess Exudates

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Author(s):
Montagner, Francisco [1] ; Jacinto, Rogerio C. [2] ; Signoretti, Fernanda G. C. ; Gomes, Brenda P. F. A. [3]
Total Authors: 4
Affiliation:
[1] Univ Fed Rio Grande do Sul, Endodont Div, Dept Conservat Dent, UFRGS, Porto Alegre, RS - Brazil
[2] Univ Fed Pelotas, Endodont Div, Pelotas, RS - Brazil
[3] Univ Estadual Campinas, Fac Odontol Piracicaba, Piracicaba Dent Sch, Dept Restorat Dent, Endodont Div, UNICAMP, BR-13414018 Piracicaba, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: JOURNAL OF ENDODONTICS; v. 36, n. 11, p. 1796-1799, NOV 2010.
Web of Science Citations: 15
Abstract

Introduction: Different microbial communities have been associated with acute endodontic infections. The majority of the microorganisms are as yet uncultivable or difficult to grow under current laboratory conditions. Treponema species are strict anaerobic bacteria that are involved in several oral diseases. The aim of this study was to detect the presence of Treponema species in infected root canals (RCs) and exudates related to acute apical abscesses (AAAs) as well as to determine positive association between targeted species and clinical signs/symptoms. Methods: Paired samples of infected RCs and AAAs were collected from 20 subjects. Nested polymerase chain reaction assay with species-specific primers for 165 rDNA and downstream intergenic spacer region was used for microbial detection. The frequency of species and statistical associations between species and signs/symptoms of endodontic origin as well as their simultaneous detection in both milieus were investigated. Results: The most frequently detected species were T. socranskii (RC, 17/20; AM, 15/20), T. denticola (RC, 8/20; AAA, 11/20); T. medium (RC, 6/20; AM, 9/20); and T. amylovorum (RC, 5/20; AM, 9/20). Positive correlation was found for simultaneous presence of I denticola in both RCs and AAAs (p = 0.01). Positive association was observed between I medium and T. vincentii (p = .037). No positive statistical association was observed between the targeted species and signs/symptoms. Conclusions: The high incidence of Treponema species in RC and AAA samples from the same tooth indicated that they are important pathogens in acute endodontic infections. (1 Endod 2010;36:1796-1799) (AU)