Doxazosin Treatment Alters Stromal Cell Behavior and Increases Elastic System Fibers Deposition in Rat Prostate

Autor(es)

Delella, Flavia Karina ^{[1, 2]} ; Felisbino, Sergio Luis ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Inst Biociencias, Dept Morfol, BR-18618000 Botucatu, SP - Brazil

^[2] Univ Estadual Campinas, Inst Biol, Dept Cell Biol, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MICROSCOPY RESEARCH AND TECHNIQUE; v. 73, n. 11, p. 1036-1044, OCT 2010.
• Citações Web of Science: 5

Resumo

Doxazosin (DOX), an alpha-adrenoceptor antagonist, induces the relaxation of smooth muscle cell tonus and reduces the clinical symptoms of benign prostatic hyperplasia (BPH). However, the effects of DOX in the prostate stromal microenvironment are not fully known. In a previous study, we showed that DOX treatment for 30 days increased deposition of collagen fibers in the three rat prostatic lobes. Herein, we investigated the effects of DOX on stromal cell ultrastructure and elastic fiber deposition. Adult Wistar rats were treated with DOX (25 mg/kg/day); and the ventral, dorsal, and anterior prostates were excised at 30 days of treatment. The prostatic lobes were submitted to histochemical and stereological-morphometric analyze and transmission electron microscopy (TEM). Histochemical staining plus stereological analysis of the elastic fiber system showed that DOX-treated prostatic lobes presented more elaunin and elastic fibers than controls, mainly in the ventral lobe. Ultrastructural analysis showed that fibroblasts and smooth muscle cells from DOX-treated prostates presented active synthetic phenotypes, evidenced by enlarged rough endoplasmic reticulum and Golgi apparatus cisterns, and confirmed the observation of thickened elaunin fibers. Our findings suggest that, under alpha-adrenergic blockade by DOX, the fibroblasts become more active and smooth muscle cells shift from a predominantly contractile to a more synthetic phenotype. The deposition of collagen and elastic system fibers in the prostatic stroma may counterbalance the absence of smooth muscle tone during alpha-blockers treatment. Microsc. Res. Tech. 73: 1036-1044, 2010. (C) 2010 Wiley-Liss, Inc. (AU)

• Processo FAPESP: 06/60114-6 - Efeito da finasterida e da doxazosina sobre os diferentes lobos da prostata de rato: analise ultraestrutural e da expressao dos colagenos tipo i e iii e do tgf-beta 1.
• Beneficiário: Flávia Karina Delella
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 06/60115-2 - Efeito do diabete e da reposicao com insulina sobre o processo de maturacao da prostata ventral do rato wistar: alteracoes morfologicas e na atividade de metaloproteinases de matriz mmp-2 e -9.
• Beneficiário: Sérgio Luis Felisbino
• Modalidade de apoio: Auxílio à Pesquisa - Regular