DNA Methylation Status of the IL8 Gene Promoter in Aggressive Periodontitis

Autor(es)

Andia, Denise C. ^[1] ; de Oliveira, Naila F. P. ^[1] ; Casarin, Renato C. V. ^[2] ; Casati, Marcio Z. ^[2] ; Line, Sergio R. P. ^[1] ; de Souza, Ana P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Dent Piracicaba, Mol Biol Lab, Dept Morphol, Div Histol, BR-13414018 Piracicaba, SP - Brazil

^[2] Univ Estadual Campinas, Sch Dent Piracicaba, Div Periodont, Dept Prosthodont & Periodont, BR-13414018 Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Periodontology; v. 81, n. 9, p. 1336-1341, SEP 2010.
• Citações Web of Science: 31

Resumo

Background: Studies evaluating the methylation status of cytokine genes may have relevance for inflammatory diseases in which the expression of some cytokines is altered, such as periodontitis. This study observes the DNA methylation status in the interleukin-8 (IL8) gene promoter in cells of the oral epithelium of subjects affected by generalized aggressive periodontitis (AgP) and compares it to those of control subjects. Methods: Genomic DNA from epithelial oral cells of 37 generalized AgP patients and 37 controls were purified and modified by sodium bisulphite. Modified DNA was submitted by methylation-specific polymerase chain reaction, electrophoresed on 10% polyacrylamide gels, and stained. Results: Subjects who presented generalized AgP have a higher frequency of hypomethylation of the IL8 gene promoter in oral epithelium cells than that of controls (86.5% in the generalized AgP group versus 62% in the control group; P = 0.016; chi(2) test). Conclusions: A marked hypomethylated status is found in the oral epithelial cells of subjects presenting with generalized AgP, compared to controls, in the promoter region of the IL8 gene. This hypomethylated status may reflect a generalized condition of oral epithelial cells, including gingival epithelium, because gingival epithelial cells were also collected during mouthwash use. J Periodontol 2010;81:1336-1341. (AU)

• Processo FAPESP: 07/02488-0 - Análise de metilação no promotor dos genes da IL-8, iNOS, TIMP-2 e TIMP-3 em indivíduos fumantes e não-fumantes com periodontite crônica: associação com a expressão gênica destes genes no tecido gengival
• Beneficiário: Ana Paula de Souza
• Modalidade de apoio: Auxílio à Pesquisa - Regular