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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

DNA Methylation Status of the IL8 Gene Promoter in Aggressive Periodontitis

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Author(s):
Andia, Denise C. [1] ; de Oliveira, Naila F. P. [1] ; Casarin, Renato C. V. [2] ; Casati, Marcio Z. [2] ; Line, Sergio R. P. [1] ; de Souza, Ana P. [1]
Total Authors: 6
Affiliation:
[1] Univ Estadual Campinas, Sch Dent Piracicaba, Mol Biol Lab, Dept Morphol, Div Histol, BR-13414018 Piracicaba, SP - Brazil
[2] Univ Estadual Campinas, Sch Dent Piracicaba, Div Periodont, Dept Prosthodont & Periodont, BR-13414018 Piracicaba, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Periodontology; v. 81, n. 9, p. 1336-1341, SEP 2010.
Web of Science Citations: 31
Abstract

Background: Studies evaluating the methylation status of cytokine genes may have relevance for inflammatory diseases in which the expression of some cytokines is altered, such as periodontitis. This study observes the DNA methylation status in the interleukin-8 (IL8) gene promoter in cells of the oral epithelium of subjects affected by generalized aggressive periodontitis (AgP) and compares it to those of control subjects. Methods: Genomic DNA from epithelial oral cells of 37 generalized AgP patients and 37 controls were purified and modified by sodium bisulphite. Modified DNA was submitted by methylation-specific polymerase chain reaction, electrophoresed on 10% polyacrylamide gels, and stained. Results: Subjects who presented generalized AgP have a higher frequency of hypomethylation of the IL8 gene promoter in oral epithelium cells than that of controls (86.5% in the generalized AgP group versus 62% in the control group; P = 0.016; chi(2) test). Conclusions: A marked hypomethylated status is found in the oral epithelial cells of subjects presenting with generalized AgP, compared to controls, in the promoter region of the IL8 gene. This hypomethylated status may reflect a generalized condition of oral epithelial cells, including gingival epithelium, because gingival epithelial cells were also collected during mouthwash use. J Periodontol 2010;81:1336-1341. (AU)

FAPESP's process: 07/02488-0 - Analysis of genes promotor methylation of IL-8, iNOS, TIMP-2, TIMP-3 in smoking and no-smoking patients with chronic periodontitis: association with genetic transcription of these genes in gingival tissue
Grantee:Ana Paula de Souza
Support Opportunities: Regular Research Grants