Antibacterial Activity of the Essential Oil from Rosmarinus officinalis and its Major Components against Oral Pathogens

Autor(es)

Bernardes, Wagner A. ^[1] ; Lucarini, Rodrigo ^[1] ; Tozatti, Marcos G. ^[1] ; Bocalon Flauzino, Luzio G. ^[1] ; Souza, Maria G. M. ^[1] ; Turatti, Isabel C. C. ^[2] ; Andrade e Silva, Marcio L. ^[1] ; Martins, Carlos H. G. ^[1] ; da Silva Filho, Ademar A. ^[1] ; Cunha, Wilson R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Franca, BR-14404600 Franca, SP - Brazil

^[2] Fac Ciencias Farmaceut Ribeirao Preto USP, Dept Quim & Fis, BR-14040903 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES; v. 65, n. 9-10, p. 588-593, SEP-OCT 2010.
• Citações Web of Science: 31

Resumo

The essential oil of Rosmarinus officinalis L. (rosemary) was obtained by hydro-distillation and analysed by gas chromatography-mass spectrometry. Sixty-two constituents were identified, representing 98.06% of the total oil content. Oxygenated monoterpenes were the predominant components. The rosemary oil was characterized as having prominent (> 5%) contents of camphor (18.9%), verbenone (11.3%), alpha-pinene (9.6%), beta-myrcene (8.6%), 1,8-cineole (8.0%), and beta-caryophyllene (5.1%). The antimicrobial activity of the oil as well as of its major constituents was tested against the following microorganisms: Streptococcus mutans, Streptococcus mitts, Streptococcus sanguinis, Streptococcus salivarius, Streptococcus sobrinus, and Enterococcus faecalis, which are potentially responsible for the formation of dental caries in humans. The microdilution method was used for determination of the minimum inhibitory concentration (MIC) during evaluation of the antibacterial activity. The essential oil displayed low activity against the selected microorganisms. In the present study, the pure major compounds were more active than the essential oil. Among all the microorganisms tested, the pathogen S. mills was the most susceptible and E. faecalis was the most resistant to the evaluated samples. This is the first report on antimicrobial activity of the major components of rosemary oil against oral pathogens. (AU)

• Processo FAPESP: 09/00604-8 - Moléculas de interesse: isolamento, identificação, modificações estruturais e avaliação em diversos ensaios biológicos
• Beneficiário: Wilson Roberto Cunha
• Modalidade de apoio: Auxílio à Pesquisa - Regular