The Detection of in Vivo and in Vitro HIV Type 1 B/F Profiles in Brazil Using a Real-Time PCR Assay for Five HIV Type 1 Genomic Regions

Autor(es)

Teixeira, Daniela ^[1] ; Munerato, Patricia ^[1] ; Vasconcelos Komninakis, Shirley Cavalcante ^[1] ; Fusuma, Erika Etsuko ^[1] ; Janini, Luiz Mario ^[1] ; Araripe Sucupira, Maria Cecilia ^[1] ; Diaz, Ricardo Sobhie ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Paulista Sch Med, Retrovirol Lab, BR-04039032 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: AIDS Research and Human Retroviruses; v. 26, n. 9, p. 981-990, SEP 2010.
• Citações Web of Science: 4

Resumo

We sought to determine the frequency and profile of HIV-1 BF recombinants in vitro and in vivo. Laboratory HIV-1 strains from subtypes B and F were cocultured and evaluated. Clinical samples from the city of Santos, Brazil, where the first HIV-1 B/F circulating recombinant forms (CRF) were described, were also assessed. Five real-time PCR assays were developed to equally amplify subtypes B and F, and subtype-specific probes were developed and optimized. To validate the PCR systems, clinical samples from Santos were sequenced and phylogenetically analyzed. The real-time PCR assays were performed on these samples and on the supernatant of an in vitro competition assay to assess emergent recombinant strains. Out of 157 clinical samples, 62.1% were defined as subtype B, 3.0% were subtype F, 16.7% presented the CRF28\_BF profile, and 13.6% of the samples presented the CRF29\_BF profile. The specificity and sensitivity in the discrimination assay for this sample panel were 93% and 92%, respectively. The HIV that emerged from the coinfected cell culture closely resembled the CRF28\_BF profile. The first-described CRFs are still fixed in this geographic region of Brazil, and the in vitro emerging strains detected by real-time PCR suggest that in addition to the shaping of recombinant strains by immune selection, viral structures may also play an important role in emerging CRFs. (AU)

• Processo FAPESP: 04/15856-9 - Análise prospectiva das características virológicas e imunológicas em indivíduos com infecção recente pelo HIV-1 das cidades de São Paulo e Santos, SP
• Beneficiário: Ricardo Sobhie Diaz
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 06/52722-6 - Otimização de técnica de PCR em tempo real para detecção das regiões pol (protease) e env dos subtipos B e F de HIV-1 e triagem dos seus recombinantes
• Beneficiário: Daniela Teixeira
• Modalidade de apoio: Bolsas no Brasil - Mestrado