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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The Detection of in Vivo and in Vitro HIV Type 1 B/F Profiles in Brazil Using a Real-Time PCR Assay for Five HIV Type 1 Genomic Regions

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Author(s):
Teixeira, Daniela [1] ; Munerato, Patricia [1] ; Vasconcelos Komninakis, Shirley Cavalcante [1] ; Fusuma, Erika Etsuko [1] ; Janini, Luiz Mario [1] ; Araripe Sucupira, Maria Cecilia [1] ; Diaz, Ricardo Sobhie [1]
Total Authors: 7
Affiliation:
[1] Univ Fed Sao Paulo, Paulista Sch Med, Retrovirol Lab, BR-04039032 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: AIDS Research and Human Retroviruses; v. 26, n. 9, p. 981-990, SEP 2010.
Web of Science Citations: 4
Abstract

We sought to determine the frequency and profile of HIV-1 BF recombinants in vitro and in vivo. Laboratory HIV-1 strains from subtypes B and F were cocultured and evaluated. Clinical samples from the city of Santos, Brazil, where the first HIV-1 B/F circulating recombinant forms (CRF) were described, were also assessed. Five real-time PCR assays were developed to equally amplify subtypes B and F, and subtype-specific probes were developed and optimized. To validate the PCR systems, clinical samples from Santos were sequenced and phylogenetically analyzed. The real-time PCR assays were performed on these samples and on the supernatant of an in vitro competition assay to assess emergent recombinant strains. Out of 157 clinical samples, 62.1% were defined as subtype B, 3.0% were subtype F, 16.7% presented the CRF28\_BF profile, and 13.6% of the samples presented the CRF29\_BF profile. The specificity and sensitivity in the discrimination assay for this sample panel were 93% and 92%, respectively. The HIV that emerged from the coinfected cell culture closely resembled the CRF28\_BF profile. The first-described CRFs are still fixed in this geographic region of Brazil, and the in vitro emerging strains detected by real-time PCR suggest that in addition to the shaping of recombinant strains by immune selection, viral structures may also play an important role in emerging CRFs. (AU)

FAPESP's process: 04/15856-9 - Prospective analysis of the virological and immunological characteristics in individuals with recent HIV-1 infection in the cities of São Paulo and Santos
Grantee:Ricardo Sobhie Diaz
Support Opportunities: Research Projects - Thematic Grants