Short-term specialized enteral diet fails to attenuate malnutrition impairment of experimental open wound acute healing

Autor(es)

Alves, Claudia Cristina ^[1] ; Torrinhas, Raquel Susana ^[2] ; Giorgi, Ricardo ^[3] ; Brentani, Maria Mitzi ^[4] ; Logullo, Angela Flavia ^[5] ; Arias, Victor ^[5] ; Mauad, Thais ^[6] ; Ferraz da Silva, Luiz Fernando ^[6] ; Waitzberg, Dan L. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Hlth Sci, Santos - Brazil

^[2] Univ Sao Paulo, Sch Med, Dept Gastroenterol, Metanutri Team, Lab Nutr & Metab Surg Digest Syst LIM 35, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Med, Dept Internal Med, Endocrinol Discipline, Human Nutr & Metab Dis Lab LIM 25, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Sch Med, Dept Radiol, Expt Oncol Dept LIM 24, Sao Paulo - Brazil

^[5] Univ Fed Sao Paulo, Dept Pathol, Sao Paulo - Brazil

^[6] Univ Sao Paulo, Sch Med, Dept Pathol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: NUTRITION; v. 26, n. 9, p. 873-879, SEP 2010.
• Citações Web of Science: 5

Resumo

Objective: We assessed the effect of enteral refeeding on the morphology, gene expression, and contraction of acute open wounds in previously malnourished rats using two different enteral diets. Methods: Adult male isogenic Lewis rats divided into two groups (eutrophic, n = 30; and previously malnourished, 12-15% body weight loss, n = 27) were subjected to cutaneous dorsal wounds and gastrostomy. Control rats received a standard oral diet (AIN-93M chow) plus enteral saline solution. Subject rats received chow plus a standard enteral diet or an enteral diet enriched with arginine and antioxidants. On post-trauma days 7 and 14, wound granulation tissue samples were collected for morphologic analysis using hematoxylin and eosin and picrosirius stain or immunohistochemistry slides and real-time polymerase chain reaction for collagen I and III gene expression. Wound contraction was also evaluated by comparing wound images from days 0,7, and 14. Results: Malnourished control rats had increased intensity and duration of wound inflammation, impaired increase of fibroblast cells contingent on post-trauma days 7 to 14, decreased expression of collagen III, and less wound contraction compared with eutrophic control rats. A specialized enteral diet did not improve wound healing of malnourished rats but did promote wound contraction at post-trauma day 7 in eutrophic rats. Conclusion: Short-term enteral refeeding, even with a specialized diet, failed to protect previously wounded malnourished rats from a prolonged inflammatory phase and impaired healing. (C) 2010 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 05/54185-5 - Influencia de dieta enteral suplementada com arginina e antioxidantes sobre cicatrizacao cutanea em ratos desnutridos.
• Beneficiário: Dan Linetzky Waitzberg
• Modalidade de apoio: Auxílio à Pesquisa - Regular