| Full text | |
| Author(s): |
Alves, Claudia Cristina
[1]
;
Torrinhas, Raquel Susana
[2]
;
Giorgi, Ricardo
[3]
;
Brentani, Maria Mitzi
[4]
;
Logullo, Angela Flavia
[5]
;
Arias, Victor
[5]
;
Mauad, Thais
[6]
;
Ferraz da Silva, Luiz Fernando
[6]
;
Waitzberg, Dan L.
[2]
Total Authors: 9
|
| Affiliation: | [1] Univ Fed Sao Paulo, Dept Hlth Sci, Santos - Brazil
[2] Univ Sao Paulo, Sch Med, Dept Gastroenterol, Metanutri Team, Lab Nutr & Metab Surg Digest Syst LIM 35, Sao Paulo - Brazil
[3] Univ Sao Paulo, Sch Med, Dept Internal Med, Endocrinol Discipline, Human Nutr & Metab Dis Lab LIM 25, Sao Paulo - Brazil
[4] Univ Sao Paulo, Sch Med, Dept Radiol, Expt Oncol Dept LIM 24, Sao Paulo - Brazil
[5] Univ Fed Sao Paulo, Dept Pathol, Sao Paulo - Brazil
[6] Univ Sao Paulo, Sch Med, Dept Pathol, Sao Paulo - Brazil
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | NUTRITION; v. 26, n. 9, p. 873-879, SEP 2010. |
| Web of Science Citations: | 5 |
| Abstract | |
Objective: We assessed the effect of enteral refeeding on the morphology, gene expression, and contraction of acute open wounds in previously malnourished rats using two different enteral diets. Methods: Adult male isogenic Lewis rats divided into two groups (eutrophic, n = 30; and previously malnourished, 12-15% body weight loss, n = 27) were subjected to cutaneous dorsal wounds and gastrostomy. Control rats received a standard oral diet (AIN-93M chow) plus enteral saline solution. Subject rats received chow plus a standard enteral diet or an enteral diet enriched with arginine and antioxidants. On post-trauma days 7 and 14, wound granulation tissue samples were collected for morphologic analysis using hematoxylin and eosin and picrosirius stain or immunohistochemistry slides and real-time polymerase chain reaction for collagen I and III gene expression. Wound contraction was also evaluated by comparing wound images from days 0,7, and 14. Results: Malnourished control rats had increased intensity and duration of wound inflammation, impaired increase of fibroblast cells contingent on post-trauma days 7 to 14, decreased expression of collagen III, and less wound contraction compared with eutrophic control rats. A specialized enteral diet did not improve wound healing of malnourished rats but did promote wound contraction at post-trauma day 7 in eutrophic rats. Conclusion: Short-term enteral refeeding, even with a specialized diet, failed to protect previously wounded malnourished rats from a prolonged inflammatory phase and impaired healing. (C) 2010 Elsevier Inc. All rights reserved. (AU) | |