Screening of Filamentous Fungi to Identify Biocatalysts for Lupeol Biotransformation

Autor(es)

de Carvalho, Tatiane C. ^[1] ; Polizeli, Aline M. ^[1] ; Turatti, Izabel C. C. ^[1] ; Severiano, Marcela E. ^[2] ; de Carvalho, Carlos E. ^[2] ; Ambrosio, Sergio R. ^[2] ; Crotti, Antonio E. M. ^[2] ; de Figueiredo, Uir S. ^[3] ; Vieira, Paulo C. ^[4] ; Furtado, Niege A. J. C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Franca, BR-14404600 Franca, SP - Brazil

^[3] Univ Fed Mato Grosso, Inst Ciencias Exatas & Terra, BR-78060900 Cuiaba, MT - Brazil

^[4] Univ Fed Sao Carlos, Ctr Ciencias Exatas & Tecnol, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Molecules; v. 15, n. 9, p. 6140-6151, SEP 2010.
• Citações Web of Science: 18

Resumo

The goal of the study was to evaluate the ability of filamentous fungi to biotransform the pentacyclic triterpene lupeol. The microbial transformations were carried out in shake flasks in different media. Experiments were also run with control flasks. Samples of each culture were taken every 24 hours, extracted with ethyl acetate, and analyzed by GC-MS. The biotransformation of lupeol by Aspergillus ochraceus and Mucor rouxii afforded two compounds in each culture, which were detected in the cultures developed for more than seven days only in the Koch's K1 medium. The obtained data demonstrated that A. ochraceus is a good biocatalyst to introduce double bonds in the lupeol structure, whereas M. rouxii exhibits ability to biocatalyze oxygen insertions in that pentacyclic triterpene. Mass spectrometry was demonstrated to be an efficient analytical method to select promising biocatalysts for the compound investigated in this study. The biotransformation processes were influenced by the culture medium and incubation period. The obtained results open the perspective of using A. ochraceus and M. rouxii in pentacyclic triterpene biotransformations. (AU)

• Processo FAPESP: 05/59329-5 - Estudos sobre a biotransformação de triterpenos pentacíclicos por fungos filamentosos e avaliação das atividades antitumoral e tripanocida dos derivados obtidos
• Beneficiário: Niege Araçari Jacometti Cardoso Furtado
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Jovens Pesquisadores