Astaxanthin ameliorates the redox imbalance in lymphocytes of experimental diabetic rats

Autor(es)

Otton, Rosemari ^{[1, 2]} ; Marin, Douglas Popp ^[2] ; Bolin, Anaysa Paola ^[2] ; Macedo dos Santos, Rita de Cassia ^[2] ; Polotow, Tatiana Geraldo ^[2] ; Sampaio, Sandra Coccuzzo ^[3] ; de Barros, Marcelo Paes ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Cruzeiro Sul, CBS, Postgrad Program, BR-08060070 Sao Paulo - Brazil

^[2] Univ Cruzeiro Sul, Inst Phys Act & Sport Sci, Postgrad Program, BR-01506000 Sao Paulo - Brazil

^[3] Butantan Inst, Sci Dev Div, Lab Physiopathol, BR-05503900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Chemico-Biological Interactions; v. 186, n. 3, p. 306-315, AUG 5 2010.
• Citações Web of Science: 38

Resumo

Diabetes mellitus is a syndrome of impaired insulin secretion/sensitivity and frequently diagnosed by hyperglycemia, lipid abnormalities, and vascular complications. The diabetic `glucolipotoxicity' also induces immunodepression in patients by redox impairment of immune cells. Astaxanthin (ASTA) is a pinkish-orange carotenoid found in many marine foods (e.g. shrimp, crabs, salmon), which has powerful antioxidant, photoprotective, antitumor, and cardioprotective properties. Aiming for an antioxidant therapy against diabetic immunodepression, we here tested the ability of prophylactic ASTA supplementation (30 days, 20 mg ASTA/kg BW) to oppose the redox impairment observed in isolated lymphocytes from alloxan-induced diabetic Wistar rats. The redox status of lymphocytes were thoroughly screened by measuring: (i) production of superoxide (O(2)(center dot-)), nitric oxide (NO(center dot)), and hydrogen peroxide (H(2)O(2)); (ii) cytosolic Ca(2+); (iii) indexes of oxidative injury; and (iv) activities of major antioxidant enzymes. Hypolipidemic and antioxidant effects of ASTA in plasma of ASTA-fed/diabetic rats were apparently reflected in the circulating lymphocytes, since lower activities of catalase, restored ratio between glutathione peroxidase and glutathione reductase activities and lower scores of lipid oxidation were concomitantly measured in those immune cells. Noteworthy, lower production of NO(center dot) and O(2)(center dot-) (precursors of peroxynitrite), and lower cytosolic Ca(2+) indicate a hypothetical antiapoptotic effect of ASTA in diabetic lymphocytes. However, questions are still open regarding the proper ASTA supplementation dose needed to balance efficient antioxidant protection and essential NO(center dot)/H(2)O(2)-mediated proliferative capacities of diabetic lymphocytes. (C) 2010 Published by Elsevier Ireland Ltd. (AU)

• Processo FAPESP: 02/09405-9 - Estudo da atividade antioxidante da astaxantina em lipossomos unilamelares: bloqueando radicais livres e afetando a permeabilidade da membrana?
• Beneficiário: Marcelo Paes de Barros
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 07/03334-6 - Avaliação dos efeitos anti-oxidantes da astaxantina e seu papel regulador na funcionalidade de neutrófilos e linfócitos de diabéticos
• Beneficiário: Rosemari Otton
• Modalidade de apoio: Auxílio à Pesquisa - Regular