| Texto completo | |
| Autor(es): |
Andrade, Valeria C. C.
;
Vettore, Andre L.
[1]
;
Panepucci, Rodrigo A.
[2]
;
Almeida, Manuella S. S.
;
Yamamoto, Mihoko
;
De Carvalho, Fabricio
;
Caballero, Otavia L.
[3]
;
Zago, Marco Antonio
[3]
;
Colleoni, Gisele W. B.
[4]
Número total de Autores: 9
|
| Afiliação do(s) autor(es): | [1] Univ Fed Sao Paulo, Dept Biol, Diadema - Brazil
[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Sao Paulo - Brazil
[3] Ludwig Inst Canc Res, New York Branch, New York, NY - USA
[4] Univ Fed Sao Paulo, EPM, Discipline Hematol & Hemotherapy, BR-04023900 Sao Paulo - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Leukemia & Lymphoma; v. 51, n. 8, p. 1543-1549, AUG 2010. |
| Citações Web of Science: | 3 |
| Resumo | |
Considering that the importance of cancer/testis (CT) antigens in multiple myeloma (MM) biology is still under investigation, the present study aimed to: (1) identify genes differentially expressed in MM using microarray analysis of plasma cell samples, separated according to the number of expressed CTs; (2) examine possible pathways related to MM pathogenesis; (3) validate the expression of candidate genes by quantitative real-time PCR (RQ-PCR). Three samples predominantly positive (>6 expressed), including the U266 cell line, and three samples predominantly negative (0 or 1 expressed CT for the 13 analyzed CT antigens), were submitted for microarray analysis. Validation by RQ-PCR from 24 MM samples showed that the ITGAS gene was downregulated in predominantly positive (>6 expressed CTs, p = 0.0030) and in tumor versus normal plasma cells (p = 0.0182). The RhoD gene was overexpressed in tumor plasma cells when compared to normal plasma cells (p = 0.0339). Results of the microarray analysis corroborate the hypothesis that MM could be separated into predominantly positive and predominantly negative expression. The differential expression of ITGA5 and RhoD suggests disruption of the focal adhesion pathway in MM and offers a new target field to be explored in this disease. (AU) | |
| Processo FAPESP: | 04/12855-1 - Analise do perfil de expressao genica pela tecnica de microarrays de oligonucleotideos em subgrupos de pacientes com mieloma multiplo definidos a partir de antigenos especificos de cancer/testiculo (ctas) |
| Beneficiário: | Valeria Cristina da Costa Andrade |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 04/13213-3 - Estudos de expressao genica em mieloma multiplo: identificacao de marcadores tumorais e possiveis alvos terapeuticos. |
| Beneficiário: | Gisele Wally Braga Colleoni |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |