Immune response to cytolethal distending toxin of Aggregatibacter actinomycetemcomitans in periodontitis patients

Autor(es)

Ando, E. S. ^[1] ; De-Gennaro, L. A. ^[2] ; Faveri, M. ^{[1, 3]} ; Feres, M. ^[3] ; DiRienzo, J. M. ^[4] ; Mayer, M. P. A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, BR-05508900 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Sao Paulo - Brazil

^[3] Univ Guarulhos, Dept Periodontol, Sao Paulo - Brazil

^[4] Univ Penn, Sch Dent Med, Dept Microbiol, Philadelphia, PA 19104 - USA

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PERIODONTAL RESEARCH; v. 45, n. 4, p. 471-480, AUG 2010.
• Citações Web of Science: 14

Resumo

Background and Objective: Cytolethal distending toxin (CDT) is a genotoxin produced by Aggregatibacter actinomycetemcomitans. In spite of its association with pathogenesis, little is known about the humoral immune response against the CDT. This study aimed to test whether subgingival colonization and humoral response to A. actinomycetemcomitans would lead to a response against CDT. Material and Methods: Sera from periodontally healthy, localized and generalized aggressive periodontitis and chronic periodontitis subjects (n = 80) were assessed for immunoglobulin G titers to A. actinomycetemcomitans serotypes a/b/c and to each CDT subunit (CdtA, CdtB and CdtC) by ELISA. A. actinomycetemcomitans subgingival levels and neutralization of CDT activity were also analyzed. Results: Sera from 75.0% localized and 81.8% generalized aggressive periodontitis patients reacted to A. actinomycetemcomitans. A response to serotype b was detected in localized (66.7%) and generalized aggressive periodontitis (54.5%). Reactivity to A. actinomycetemcomitans correlated with subgingival colonization (R = 0.75, p < 0.05). There was no correlation between A. actinomycetemcomitans colonization or response to serotypes and the immunoglobulin G response to CDT subunits. Titers of immunoglobulin G to CdtA and CdtB did not differ among groups; however, sera of all generalized aggressive periodontitis patients reacted to CdtC. Neutralization of CDT was not correlated with levels of antibodies to CDT subunits. Conclusion: Response to CdtA and CdtB did not correlate with the periodontal status of the subject in the context of an A. actinomycetemcomitans infection. However, a response to CdtC was found in sera of generalized but not of localized aggressive periodontitis subjects. Differences in response to CdtC between generalized and localized aggressive periodontitis subjects indicate that CDT could be expressed differently by the infecting strains. Alternatively, the antibody response to CdtC could require the colonization of multiple sites. (AU)

• Processo FAPESP: 03/03318-0 - Toxina da distensão citoletal (CDT): análise da resposta imune humoral específica em soros de pacientes com diferentes condições periodontais e seu efeito na inibição da atividade macrofágica
• Beneficiário: Ellen Sayuri Ando Suguimoto
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 03/08598-0 - Fatores de virulência de A. actinomycetemcomitans: participação na doença, regulação da expressão, diversidade e resposta imune
• Beneficiário: Marcia Pinto Alves Mayer
• Modalidade de apoio: Auxílio à Pesquisa - Temático