Exercise at anaerobic threshold intensity and insulin secretion by isolated pancreatic islets of rats

Autor(es)

Machado de Oliveira, Camila Aparecida ^[1] ; Paiva, Mauricio Ferreira ^[2] ; Soares Mota, Clecia Alencar ^[2] ; Ribeiro, Carla ^[2] ; Curiacos de Almeida Leme, Jose Alexandre ^[2] ; Luciano, Eliete ^[2] ; Rostom de Mello, Maria Alice ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Dept Anat Cellular Biol Physiol & Biophys, Campinas, SP - Brazil

^[2] Univ Estadual Paulista, Dept Phys Educ, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ISLETS; v. 2, n. 4, p. 240-246, JUL-AUG 2010.
• Citações Web of Science: 12

Resumo

To evaluate the effect of acute exercise and exercise training at the anaerobic threshold (AT) intensity on aerobic conditioning and insulin secretion by pancreatic islets, adult male Wistar rats were submitted to the lactate minimum test (LMT) for AT determination. Half of the animals were submitted to swimming exercise training (trained), 1 h/day, 5 days/week during 8 weeks, with an overload equivalent to the AT. The other half was kept sedentary. At the end of the experimental period, the rats were submitted to an oral glucose tolerance test and to another LMT. Then, the animals were sacrificed at rest or immediately after 20 minutes of swimming exercise at the AT intensity for pancreatic islets isolation. At the end of the experiment mean workload (% bw) at AT was higher and blood lactate concentration (mmol/L) was lower in the trained than in the control group. Rats trained at the AT intensity showed no alteration in the areas under blood glucose and insulin during OGTT test. Islet insulin content of trained rats was higher than in the sedentary rats while islet glucose uptake did not differ among the groups. The static insulin secretion in response to the high glucose concentration (16.7 mM) of the sedentary group at rest was lower than the sedentary group submitted to the acute exercise and the inverse was observed in relation to the trained groups. Physical training at the AT intensity improved the aerobic condition and altered insulin secretory pattern by pancreatic islets. (AU)

• Processo FAPESP: 05/02139-0 - Modulação pelo exercício do metabolismo de glicose e proteínas em ratos diabéticos aloxânicos
• Beneficiário: Maria Alice Rostom de Mello
• Modalidade de apoio: Auxílio à Pesquisa - Regular