Changes in the frequency of the G137D and W251S mutations in the carboxylesterase E3 gene of Cochliomyia hominivorax (Diptera: Calliphoridae) populations from Uruguay

Autor(es)

de Carvalho, Renato Assis ^[1] ; Gomez Limia, Cintia Elizabeth ; Bass, Chris ^[2] ; Lima de Azeredo-Espin, Ana Maria

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, CBMEG, Lab Genet Anim, Depto Genet & Evolucao, BR-13083875 Campinas, SP - Brazil

^[2] Ctr Sustainable Pest & Dis Management, Harpenden AL5 2JQ, Herts - England

• Tipo de documento: Artigo Científico
• Fonte: Veterinary Parasitology; v. 170, n. 3-4, p. 297-301, JUN 24 2010.
• Citações Web of Science: 3

Resumo

The New World Screwworm (NWS) fly Cochliomyia hominivorax is one of most important myiasis-causing flies in the Neotropics. It is responsible for severe losses to the livestock industry through both mortality and the loss of productivity of infested animals. In Uruguay, NWS represents a significant problem. To date this pest has been controlled by the application of chemical insecticides, mainly the pyrethroid and organophosphate (OP) classes. However, the intensive use of these compounds over many years has led to the evolution of resistance which has the potential to compromise the effectiveness of current control strategies. One mechanism by which resistance has occurred in this and related dipteran species is through two mutations (G137D and W251S) in the carboxylesterase E3 enzyme that have enhanced ability to hydrolyze certain insecticides. In this study changes in the frequency of these mutations in C. hominivorax was investigated in three different Uruguayan regions in 2003 and 2009. All three regions analyzed showed a reduction in the frequency of the G137D mutation and a significant increase in frequency of the W251S mutation, and this may be related to the current intense use of dimethyl-OP and pyrethroid insecticides. The findings of this study provide current information on the frequency of these resistance-associated mutations in NWS in Uruguay and may help select appropriate chemicals for NWS control as part of potential pest management strategies. (C) 2010 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 07/54431-1 - Investigação genética da resistência a inseticidas organofosforados e piretróides na mosca praga da pecuária Cochliomya hominivorax (Diptera: Calliphoridae)
• Beneficiário: Ana Maria Lima de Azeredo-Espin
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 06/60693-6 - Identificação de genes diferencialmente expressos em linhagem da praga da pecuária Cochliomyia hominivorax (Diptera: Calliphoridae) resistente a inseticida organofosforado
• Beneficiário: Renato Assis de Carvalho
• Modalidade de apoio: Bolsas no Brasil - Doutorado