Molecular characterization of human polyomavirus JC in Brazilian AIDS patients with and without progressive multifocal leukoencephalopathy

Autor(es)

Domingues Fink, Maria Cristina ^[1] ; Penalva de Oliveira, Augusto Cesar ^[2] ; Romano, Camila Malta ^{[1, 3]} ; Vidal, Jose Ernesto ^[2] ; Palma Urbano, Paulo Roberto ^[1] ; Tateno, Adriana Fumie ^[1] ; Oliveira, Cristina Mendes ^[1] ; de Albuquerque Luna, Expedito Jose ^[1] ; Pannuti, Claudio Sergio ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Med Trop Sao Paulo, BR-05403000 Sao Paulo - Brazil

^[2] Inst Infectol Emilio Ribas, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Med, Dept Molestias Infecciosas & Parasitarias LIMHC, BR-05403000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Clinical Virology; v. 48, n. 1, p. 6-10, MAY 2010.
• Citações Web of Science: 8

Resumo

Background: JC virus (JCV), the causative agent of progressive multifocal leukoencephalopathy (PML), is classified in 8 different genotypes. Previous reports have suggested a positive association between specific genotypes and PML. Objective: To compare genotypes and adaptive mutations of JCV strains from Brazilian AIDS patients with and without PML. Study design: The VP1 region of JCV was amplified by polymerase chain reaction from cerebrospinal fluid samples from 51 patients with PML and from urine samples of 47 patients with AIDS without central nervous system disease. Genotyping was done by phylogenetic analysis. Amino acid replacement and selection pressures were also investigated. Results: JCV genotype frequency distributions showed that genotypes 2 (32.7%), 1 (26.5%) and 3 (23.5%) were the most prevalent. Genotype 1 had a positive association (p < 0.0001) and genotype 3 showed an inverse association (p < 0.001) with PML. A previously undescribed point mutation at residue 91 (L/I or L/V) and (L/P), non-genotype-associated, was found in 5/49 (10.2%) and 2/47 (4.3%) JCV sequences from PML and non-PML patients, respectively. This mutation was under positive selection only in PML patients. A previously described substitution of T-A in position 128 showed a significant difference between PML and non-PML cases (70% versus 16%, respectively, p < 0.0005). Conclusion: In Brazilian patients with AIDS, JCV genotype 1 showed a strong association with PML (p < 0.0001) and JCV genotype 3 showed an inverse association with PML. The possible association of aminoacids substitution in residues 91 and 128 with PML in patients with AIDS must be further investigated. (C) 2010 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 07/04681-1 - Caracterização molecular do poliomavírus humano JC em pacientes com AIDS, com e sem leucoencefalopatia multifocal progressiva, em São Paulo, Brasil
• Beneficiário: Claudio Sergio Pannuti
• Modalidade de apoio: Auxílio à Pesquisa - Regular