FURO/CAP: A protocol for sodium intake sensitization

Autor(es)

Pereira, Daniela T. B. ^[1] ; Menani, Jose V. ^[1] ; De Luca, Jr., Laurival A. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Dept Physiol & Pathol, Sch Dent, BR-14801903 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Physiology & Behavior; v. 99, n. 4, p. 472-481, MAR 30 2010.
• Citações Web of Science: 21

Resumo

We investigated if a history of FURO/CAP, a protocol that increases brain angiotensin II (ANG II), sensitizes or enhances sodium intake. A subcutaneous injection of the diuretic furosemide (FURO, 10 mg/kg) was combined with a converting enzyme inhibitor captopril (CAP, 5 mg/kg) to induce a short latency stimulated sodium (0.3 M NaCl) and water intake in a 2 h FURO/CAP test. Repeated injections of only FURO/CAP, separated by one-week interval, enhanced stimulated and spontaneous (daily) sodium intake. Stimulated fluid intake was completely suppressed when FURO/CAP was combined with two intraperitoneal injections of the ANG II type-1 receptor antagonist losartan (10, 20, or 40 mg/kg each) given within 1 h prior to the FURO/CAP test. Losartan reduced by only 35% the FURO/CAP-induced natriuresis. A history of FURO/CAP, FURO/CAP + losartan (all doses), or vehicle produced similar stimulated fluid intake when all animals received only FURO/CAP in the third final FURO/CAP test. However, a history of vehicle or FURO/CAP + losartan 10 mg/kg precluded the enhancement in spontaneous sodium intake after the third final FURO/CAP. The FURO/CAP combined with losartan (all doses) also precluded the spontaneous sodium intake enhancement in the weeks that preceded the third final FURO/CAP test. A history of only FURO/CAP, but not vehicle, also enhanced water deprivation-induced sodium appetite. The results suggest that a history of FURO/CAP enhances stimulated and spontaneous sodium intake, as well as water deprivation-induced sodium appetite, and reinforce the role of ANG II as a peptide that mediates long-term effects on behavior. (C) 2009 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 03/11268-2 - Alterações de longo prazo no apetite ao sódio: aspectos comparativos, neuroendócrinos e genômicos
• Beneficiário: Daniela de Toledo Borba Pereira
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto