Effect of aging and oral tolerance on dendritic cell function

Autor(es)

Simioni, P. U. ; Fernandes, L. G. R. ; Gabriel, D. L. ; Tamashiro, W. M. S. C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Microbiol & Imunol, BR-13083862 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Medical and Biological Research; v. 43, n. 1, p. 68-76, JAN 2010.
• Citações Web of Science: 4

Resumo

Oral tolerance can be induced in some mouse strains by gavage or spontaneous ingestion of dietary antigens. In the present study, we determined the influence of aging and oral tolerance on the secretion of co-stimulatory molecules by dendritic cells (DC), and on the ability of DC to induce proliferation and cytokine secretion by naive T cells from BALB/c and OVA transgenic (DO11.10) mice. We observed that oral tolerance could be induced in BALB/c mice (N = 5 in each group) of all ages (8, 20, 40, 60, and 80 weeks old), although a decline in specific antibody levels was observed in the sera of both tolerized and immunized mice with advancing age (40 to 80 weeks old). DC obtained from young, adult and middle-aged (8, 20, and 40 weeks old) tolerized mice were less efficient (65, 17 and 20%, respectively) than DC from immunized mice (P < 0.05) in inducing antigen-specific proliferation of naive T cells from both BALB/c and DO11.10 young mice, or in stimulating IFN-g, IL-4 and IL-10 production. However, TGF-β levels were significantly elevated in co-cultures carried out with DC from tolerant mice (P < 0.05). DC from both immunized and tolerized old and very old (60 and 80 weeks old) mice were equally ineffective in inducing T cell proliferation and cytokine production (P < 0.05). A marked reduction in CD86+ marker expression was observed in DC isolated from both old and tolerized mice (75 and 50%, respectively). The results indicate that the aging process does not interfere with the establishment of oral tolerance in BALB/c mice, but reduces DC functions, probably due to the decline of the expression of the CD86 surface marker. (AU)

• Processo FAPESP: 05/03507-2 - A modulação da resposta imune de camundongos DO11.10 por transferência adotiva de células dendríticas diferenciadas in vitro
• Beneficiário: Patricia Ucelli Simioni
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 01/08462-6 - O papel das células dendríticas na indução da tolerância oral ao longo da vida de camundongos: alterações funcionais e fenotípicas
• Beneficiário: Wirla Maria da Silva Cunha Tamashiro
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 06/60128-7 - Adequação da infra estrutura para o desenvolvimento de projetos de pesquisa e de pós doutoramento na área de imunologia, com ênfase em tolerância oral
• Beneficiário: Patricia Ucelli Simioni
• Modalidade de apoio: Auxílio à Pesquisa - Programa Primeiros Projetos
• Processo FAPESP: 05/51520-8 - Tolerancia oral: geracao e funcao de metabolitos da l-arginina em celulas dendriticas.
• Beneficiário: Wirla Maria da Silva Cunha Tamashiro
• Modalidade de apoio: Auxílio à Pesquisa - Regular