| Texto completo | |
| Autor(es): |
Siqueira, Adriane S.
[1]
;
Gama-de-Souza, Leticia N.
[1, 2]
;
Arnaud, Maria Vanda C.
[3]
;
Pinheiro, Joao J. V.
[1, 4]
;
Jaeger, Ruy G.
[1, 5]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Dept Cell & Dev Biol, Inst Biomed Sci, BR-05508000 Sao Paulo - Brazil
[2] Univ Fed Espirito Santo, Dept Morphol, Ctr Hlth Sci CCS, BR-29075910 Vitoria, ES - Brazil
[3] Ophir Loyola Canc Hosp, Dept Histopathol, BR-66062240 Belem, Para - Brazil
[4] Fed Univ Para, Dept Oral Pathol, Sch Dent, BR-66073000 Belem, Para - Brazil
[5] Univ Sao Paulo, Dept Biol Celular & Desenvolvimento, Inst Ciencias Biomed, BR-05508000 Sao Paulo - Brazil
Número total de Afiliações: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | TUMOR BIOLOGY; v. 31, n. 1, p. 46-58, JAN 2010. |
| Citações Web of Science: | 20 |
| Resumo | |
Squamous cell carcinoma is a prevalent head and neck tumor with high mortality. We studied the role played by laminin alpha 1 chain peptide AG73 on migration, invasion, and protease activity of cells (OSCC) from human oral squamous cell carcinoma. Immunohistochemistry and immunofluorescence analyzed expression of laminin alpha 1 chain and MMP9 in oral squamous cells carcinoma in vivo and in vitro. Migratory activity of AG73-treated OSCC cells was investigated by monolayer wound assays and in chemotaxis chambers. AG73-induced invasion was assessed in Boyden chambers. Invasion depends on MMPs. Conditioned media from cells grown on AG73 was subjected to zymography. We searched for AG73 receptors related to these activities in OSCC cells. Immunofluorescence analyzed AG73induced colocalization of syndecan-1 and beta 1 integrin. Cells had these receptors silenced by siRNA, followed by treatment with AG73 and analysis of migration, invasion, and protease activity. Oral squamous cell carcinoma expresses laminin alpha 1 chain and MMP9. OSCC cells treated with AG73 showed increased migration, invasion, and protease activity. AG73 induced colocalization of syndecan-1 and beta 1 integrin. Knockdown of these receptors decreased AG73-dependent migration, invasion, and protease activity. Syndecan-1 and beta 1 integrin signaling downstream of AG73 regulate migration, invasion, and MMP production by OSCC cells. (AU) | |
| Processo FAPESP: | 06/57079-4 - Papel de peptídeos bioativos da laminina na migração e invasão de linhagens celulares derivadas de neoplasias de glândulas salivares |
| Beneficiário: | Ruy Gastaldoni Jaeger |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 07/51950-8 - Papel de peptídeos bioativos da laminina na migração e invasão de linhagens celulares derivadas de carcinoma epidermóide |
| Beneficiário: | Adriane Sousa de Siqueira |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 08/57103-8 - Dinamica de formacao de invadopodios em linhagens tumorais tratadas por peptideos bioativos da laminina. estudo por microscopia 4d |
| Beneficiário: | Ruy Gastaldoni Jaeger |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 05/55602-9 - Papel dos peptídeos bioativos da laminina na migração e invasão de linhagens celulares derivadas de neoplasias de glândulas salivares |
| Beneficiário: | Letícia Nogueira da Gama de Souza |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |