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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Laminin-derived peptide AG73 regulates migration, invasion, and protease activity of human oral squamous cell carcinoma cells through syndecan-1 and beta 1 integrin

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Author(s):
Siqueira, Adriane S. [1] ; Gama-de-Souza, Leticia N. [1, 2] ; Arnaud, Maria Vanda C. [3] ; Pinheiro, Joao J. V. [1, 4] ; Jaeger, Ruy G. [1, 5]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Dept Cell & Dev Biol, Inst Biomed Sci, BR-05508000 Sao Paulo - Brazil
[2] Univ Fed Espirito Santo, Dept Morphol, Ctr Hlth Sci CCS, BR-29075910 Vitoria, ES - Brazil
[3] Ophir Loyola Canc Hosp, Dept Histopathol, BR-66062240 Belem, Para - Brazil
[4] Fed Univ Para, Dept Oral Pathol, Sch Dent, BR-66073000 Belem, Para - Brazil
[5] Univ Sao Paulo, Dept Biol Celular & Desenvolvimento, Inst Ciencias Biomed, BR-05508000 Sao Paulo - Brazil
Total Affiliations: 5
Document type: Journal article
Source: TUMOR BIOLOGY; v. 31, n. 1, p. 46-58, JAN 2010.
Web of Science Citations: 20
Abstract

Squamous cell carcinoma is a prevalent head and neck tumor with high mortality. We studied the role played by laminin alpha 1 chain peptide AG73 on migration, invasion, and protease activity of cells (OSCC) from human oral squamous cell carcinoma. Immunohistochemistry and immunofluorescence analyzed expression of laminin alpha 1 chain and MMP9 in oral squamous cells carcinoma in vivo and in vitro. Migratory activity of AG73-treated OSCC cells was investigated by monolayer wound assays and in chemotaxis chambers. AG73-induced invasion was assessed in Boyden chambers. Invasion depends on MMPs. Conditioned media from cells grown on AG73 was subjected to zymography. We searched for AG73 receptors related to these activities in OSCC cells. Immunofluorescence analyzed AG73induced colocalization of syndecan-1 and beta 1 integrin. Cells had these receptors silenced by siRNA, followed by treatment with AG73 and analysis of migration, invasion, and protease activity. Oral squamous cell carcinoma expresses laminin alpha 1 chain and MMP9. OSCC cells treated with AG73 showed increased migration, invasion, and protease activity. AG73 induced colocalization of syndecan-1 and beta 1 integrin. Knockdown of these receptors decreased AG73-dependent migration, invasion, and protease activity. Syndecan-1 and beta 1 integrin signaling downstream of AG73 regulate migration, invasion, and MMP production by OSCC cells. (AU)