Assessment of the Expression of IR beta, IRS-1, IRS-2 and IGF-IR beta in a Rat Model of Intrauterine Growth Restriction

Autor(es)

Bueno, Marcia Pereira ^[1] ; Guadagnini, Dioze ^[2] ; Lanhellas Goncalves, Frances Lilian ; Barini, Ricardo ^[2] ; Abdalla Saad, Mario Jose ^[2] ; Schmidt, Augusto Frederico ; Sbragia, Lourenco ^[3]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Fac Med Sci, Dept Obstet & Gynecol, Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Fac Med Sci, Dept Internal Med, Campinas, SP - Brazil

^[3] Univ Sao Paulo, Lab Expt Fetal Surg Michael R Harrison, Dept Surg & Anat, Sch Med Ribeirao Preto, BR-14048900 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FETAL DIAGNOSIS AND THERAPY; v. 28, n. 3, p. 145-152, 2010.
• Citações Web of Science: 5

Resumo

Objective: To investigate glomerular development and expression of insulin and insulin-like growth factor receptors in an experimental model of intrauterine growth restriction (IUGR). Material and Methods: We studied three groups of Sprague-Dawley fetuses: IUGR - restricted by ligation of the right uterine artery; C-IUGR - left horn controls, and EC - external controls (non-manipulated). Body and organs were weighed, and glomerular number and volume were analyzed. Expression of IR beta, IRS-1, IRS-2 and IGF-IR beta was analyzed in liver, intestine and kidneys by immunoblotting. Results: Organ/body weight ratios were similar. In IUGR, glomerular number and volume were increased compared to C-IUGR and EC (p < 0.001). In the IUGR liver, increases were found in IGF-IR beta compared to C-IUGR and EC; IR beta compared to EC, and IRS-2 compared to C-IUGR. However, decreases in IR beta were noted in IUGR compared to C-IUGR; IRS-1 compared to C-IUGR and EC, and IRS-2 compared to EC. In IUGR intestine, increases were detected in IR beta, IRS-1 and IGF-IR beta compared to C-IUGR and EC. In IUGR kidneys, increases were observed in IR beta and IGF-IR beta compared to C-IUGR and EC, and IRS-1 compared to EC. Decreased IRS-2 in the intestine and kidney were noticed in IUGR compared to C-IUGR and EC. Conclusion: IUGR fetuses had less glomeruli and alterations in insulin receptors, which may be associated with an increased risk of disease occurrence in adulthood. Copyright (C) 2010 S. Karger AG, Basel (AU)

• Processo FAPESP: 08/51487-9 - Avaliacao da protecao das alcas intestinais fetais utilizando hidrogel (biomaterial) e oxido nitrico no modelo experimental de gastrosquise
• Beneficiário: Lourenço Sbragia Neto
• Modalidade de apoio: Auxílio à Pesquisa - Regular