Differential Shh, Bmp and Wnt gene expressions during craniofacial development in mice

Autor(es)

Silva Paiva, Katiucia Batista ^[1] ; Silva-Valenzuela, Maria das Gracas ^[1] ; Gomes Massironi, Silvia Maria ^[2] ; Ko, Gui Mi ^[3] ; Siqueira, Filipe Modolo ^[4] ; Nunes, Fabio Daumas ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Oral Pathol, Sch Dent, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Immunol, Inst Biomed Sci, Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Ctr Dev Anim Models, Sao Paulo - Brazil

^[4] Univ Fed Santa Catarina, Ctr Hlth Sci, Dept Pathol, BR-88040900 Florianopolis, SC - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ACTA HISTOCHEMICA; v. 112, n. 5, p. 508-517, 2010.
• Citações Web of Science: 17

Resumo

In this study, Bmp-4, Wnt-5a and Shh gene expressions were compared during early craniofacial development in mice by comparative non-isotopic in situ hybridization. Wild-type C57BL/6J mice were studied at various stages of embryonic development (from 8.5- to 13.5-day-old embryos - E8.5-13.5). During early odontogenesis, transcripts for Bmp-4, Shh and Wnt-5a were co-localised at the tooth initiation stage. At E8.5, Shh mRNA expression was restricted to diencephalon and pharyngeal endoderm. Before maxillae and mandible ossification, Bmp-4 and Wnt-5a signals were detected in the mesenchymal cells and around Meckel's cartilage. During palatogenesis, Shh was expressed only in the epithelium and Wnt-5a only in the mesenchyme of the elevating palatal shelves. During tongue development, Shh expression was found in mesenchyme, probably contributing to tongue miogenesis, while Wnt-5a signal was in the epithelium, possibly during placode development and papillae formation. Taken together, these findings suggest that Bmp-4, Shh and Wnt-5a gene expressions may act together on the epithelial mesenchymal interactions occurring in several aspects of the early mouse craniofacial development, such as odontogenesis, neuronal development, maxillae and mandible ossification, palatogenesis and tongue formation. (C) 2009 Elsevier GmbH. All rights reserved. (AU)

• Processo FAPESP: 97/13228-5 - Expressão diferencial de genes envolvidos no desenvolvimento craniofacial durante a formação do processo maxilar de embriões de camundongo: comparação com animal transgênico portador de deformidades craniofaciais
• Beneficiário: Fabio Daumas Nunes
• Modalidade de apoio: Auxílio à Pesquisa - Regular