The effect of thyroid hormones on the white adipose tissue gene expression of PAI-1 and its serum concentration

Autor(es)

Biz, C. ^[1] ; Oliveira, C. ^[1] ; Mattos, A. B. M. ^[1] ; Oliveira, J. ^[1] ; Ribeiro, E. B. ^[1] ; Oller do Nascimento, C. M. ^[1] ; Oyama, L. M. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Fisiol, Disciplina Fisiol Nutr, BR-04023062 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Dept Biociencias, Santos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Medical and Biological Research; v. 42, n. 12, p. 1163-1166, DEC 2009.
• Citações Web of Science: 3

Resumo

Metabolic syndrome is associated with an increased risk of developing cardiovascular diseases and plasminogen activator inhibitor 1 (PAI-1) overexpression may play a significant role in this process. A positive correlation between adipose tissue gene expression of PAI-1 and its serum concentration has been reported. Furthermore, high serum levels of thyroid hormones (T3 and T4) and PAI-1 have been observed in obese children. The present study evaluates the impact of thyroid hormone treatment on white adipose tissue PAI-1 gene expression and its serum concentration. Male Wistar rats (60 days old) were treated for three weeks with T4 (50 mu g/day, Hyper) or with saline (control). Additionally, 3T3-L1 adipocytes were treated for 24 h with T4 (100 nM) or T3 (100 nM). PAI-1 gene expression was determined by real-time PCR, while the serum concentration of PAI-1 was measured by ELISA using a commercial kit (Innovative Research, USA). Both the serum concentration of PAI-1 and mRNA levels were similar between groups in retroperitoneal and epididymal white adipose tissue. Using 3T3-L1 adipocytes, in vitro treatment with T4 and T3 increased the gene expression of PAI-1, suggesting non-genomic and genomic effects, respectively. These results demonstrate that thyroid hormones have different effects in vitro and in vivo on PAI-1 gene expression in adipocytes. (AU)

• Processo FAPESP: 06/58628-1 - Efeito dos hormônios tireoidianos e de antioxidantes na expressão gênica de PAI-1, adiponectina e IL-6: estudo in vivo e in vitro
• Beneficiário: Carolina Biz Rodrigues Silva
• Modalidade de apoio: Bolsas no Brasil - Mestrado