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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The effect of thyroid hormones on the white adipose tissue gene expression of PAI-1 and its serum concentration

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Author(s):
Biz, C. [1] ; Oliveira, C. [1] ; Mattos, A. B. M. [1] ; Oliveira, J. [1] ; Ribeiro, E. B. [1] ; Oller do Nascimento, C. M. [1] ; Oyama, L. M. [2]
Total Authors: 7
Affiliation:
[1] Univ Fed Sao Paulo, Dept Fisiol, Disciplina Fisiol Nutr, BR-04023062 Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Dept Biociencias, Santos, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Brazilian Journal of Medical and Biological Research; v. 42, n. 12, p. 1163-1166, DEC 2009.
Web of Science Citations: 3
Abstract

Metabolic syndrome is associated with an increased risk of developing cardiovascular diseases and plasminogen activator inhibitor 1 (PAI-1) overexpression may play a significant role in this process. A positive correlation between adipose tissue gene expression of PAI-1 and its serum concentration has been reported. Furthermore, high serum levels of thyroid hormones (T3 and T4) and PAI-1 have been observed in obese children. The present study evaluates the impact of thyroid hormone treatment on white adipose tissue PAI-1 gene expression and its serum concentration. Male Wistar rats (60 days old) were treated for three weeks with T4 (50 mu g/day, Hyper) or with saline (control). Additionally, 3T3-L1 adipocytes were treated for 24 h with T4 (100 nM) or T3 (100 nM). PAI-1 gene expression was determined by real-time PCR, while the serum concentration of PAI-1 was measured by ELISA using a commercial kit (Innovative Research, USA). Both the serum concentration of PAI-1 and mRNA levels were similar between groups in retroperitoneal and epididymal white adipose tissue. Using 3T3-L1 adipocytes, in vitro treatment with T4 and T3 increased the gene expression of PAI-1, suggesting non-genomic and genomic effects, respectively. These results demonstrate that thyroid hormones have different effects in vitro and in vivo on PAI-1 gene expression in adipocytes. (AU)