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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Prion Protein and Its Ligand Stress Inducible Protein 1 Regulate Astrocyte Development

Texto completo
Autor(es):
Arantes, Camila [1, 2] ; Nomiz, Regina [3] ; Lopes, Marilene H. [2] ; Hajj, Glaucia N. M. [2] ; Lima, Flavia R. S. [2] ; Martins, Vilma R. [2]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-01498 Sao Paulo - Brazil
[2] Hosp Alemao Oswaldo Cruz, Ludwig Inst Canc Res, Sao Paulo - Brazil
[3] Hosp AC Camargo Fund Antonio Prudente, Ctr Tratamento & Pesquisa, Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Glia; v. 57, n. 13, p. 1439-1449, OCT 2009.
Citações Web of Science: 42
Resumo

Prion protein (PrP(C)) interaction with stress inducible protein 1 (STI1) mediates neuronal survival and differentiation. However, the function of PrP(C) in astrocytes has not been approached. In this study, we show that STI1 prevents cell death in wild-type astrocytes in a protein kinase A-dependent manner, whereas PrP(C)-null astrocytes were not affected by STI1 treatment. At embryonic day 17, cultured astrocytes and brain extracts derived from PrP(C)-null mice showed a reduced expression of glial fibrillary acidic protein (GFAP) and increased vimentin and nestin expression when compared with wild-type, suggesting a slower rate of astrocyte maturation in PrP(C)-null animals. Furthermore, PrP(C)-null astrocytes treated with STI1 did not differentiate from a flat to a process-bearing morphology, as did wild-type astrocytes. Remarkably, STI1 inhibited proliferation of both wild-type and PrP(C)-null astrocytes in a protein kinase C-dependent manner. Taken together, our data show that PrP(C) and STI1 are essential to astrocyte development and act through distinct signaling pathways.(C) 2009 Wiley-Liss, Inc. (AU)

Processo FAPESP: 03/13189-2 - Papel da proteína prion celular em processos fisiológicos e patológicos II
Beneficiário:Vilma Regina Martins
Linha de fomento: Auxílio à Pesquisa - Temático