In Vitro Adhesion of Streptococcus sanguinis to Dentine Root Surface After Treatment with Er:Yag Laser, Ultrasonic System, or Manual Curette

Autor(es)

Ota-Tsuzuki, Claudia ^[1] ; Martins, Femanda L. ^[1] ; Giorgetti, Ana Paula O. ^[2] ; de Freitas, Patricia M. ^[3] ; Duarte, Poliana M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Guarulhos, Dept Periodont, Dent Res Div, Sao Paulo - Brazil

^[2] Univ Campinas Piracicaba, Dept Prosthodont Periodont, Div Periodont, Sch Dent Piracicaba, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Dent USP, Dept Restorat Dent, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Photomedicine and Laser Surgery; v. 27, n. 5, p. 735-741, OCT 2009.
• Citações Web of Science: 14

Resumo

Objective: The purpose of this in vitro study was to evaluate the dentine root surface roughness and the adherence of Streptococcus sanguinis (ATCC 10556) after treatment with an ultrasonic system, Er:YAG laser, or manual curette. Background Data: Bacterial adhesion and formation of dental biofilm after scaling and root planing may be a challenge to the long-term stability of periodontal therapy. Materials and Methods: Forty flattened bovine roots were randomly assigned to one of the following groups: ultrasonic system (n = 10); Er:YAG laser (n = 10); manual curette (n = 10); or control untreated roots (n = 10). The mean surface roughness (Ra, mu m) of the specimens before and after exposure to each treatment was determined using a surface profilometer. In addition, S. sanguinis was grown on the treated and untreated specimens and the amounts of retained bacteria on the surfaces were measured by culture method. Results: All treatments increased the Ra; however, the roughest surface was produced by the curettes. In addition, the specimens treated with curettes showed the highest S. sanguinis adhesion. There was a significant positive correlation between roughness values and bacterial cells counts. Conclusion: S. sanguinis adhesion was the highest on the curette-treated dentine root surfaces, which also presented the greatest surface roughness. (AU)

• Processo FAPESP: 97/10823-0 - Projeto Er: YAG laser em odontologia para pesquisas laboratoriais e clínicas
• Beneficiário: Carlos de Paula Eduardo
• Modalidade de apoio: Auxílio à Pesquisa - Programa Infraestrutura - Equipamentos
• Processo FAPESP: 04/01175-0 - Efeito in vitro e in situ de diferentes sistemas clareadores caseiros sobre a microdureza do esmalte dental humano e efetividade clínica
• Beneficiário: José Augusto Rodrigues
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 05/02561-3 - Perfis microbiológico e de expressão de citocinas pró- e anti-inflamatórias associados às diferentes severidades da perimplantite: estudo em humanos
• Beneficiário: Poliana Mendes Duarte
• Modalidade de apoio: Auxílio à Pesquisa - Regular